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Physiological Signiticance of Phospholipase A_2 activation in neuronal activity.

Research Project

Project/Area Number 07660407
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionOsaka Pretecture University

Principal Investigator

TAKEUCHI Tadayoshi  Osaka Prefecture University, Agriculture, Assistant Professor, 農学部, 助教授 (00171611)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPhospholipase A_2 / Acetylcholine Release / Ca^<2+>-independent fusion / Guinea Pig Myenteric Plexus / Rat Brain Synaptosome / Auto, inhibition / Protein Kinase C / Arachidonate Metabolism / モルモット回腸壁内神経常 / Protein kinase C / プロテインキナーゼA / cyclic AMP / ラット唾液腺 / 膜融合 / アミラーゼ分泌
Research Abstract

The results obtained from this study are as follows ; an activation of protein kinase A is essential for modulation of the agonist-induced release of acetylcholine (Ach) from myenteric plexus of guinea pig ileum, and the activity of protein kinase A is regulated by prostaglandins, which was synthesized from arachidonic acid released by phospholipase A_2(PLA_2), via intracellular cyclic AMP level in myenteric neurons. In addition, an inhibition of Ach release by activation of muscarnic autoreceptor on presynaptic membranes is attributed in part to an depression of phospholipase C-protein kinase C system. The Ach releases induced by electrical field stimulation in the presence of atropine (under the autoinhibition-blocked condition) or by an activation of protein kinase C were inhibited significantly by an inhibitor of PLA_2, suggesting that protein kinase C may regulate the activity of PLA_2. These results indicate that the activity of PLA_2 and metabolites of arachidonic acid are impor … More tant factors in neurotransmitter-releasing mechanisms, and the regulation of Ach release with physiological active substances may be due to an action on its enzyme.
In another experiment, synaptic vesicle and presynaptic membrane were prepared from rat brain. Synaptic vesicle fused with presynaptic membrane which had been pretreated with PLA_2 in the presence of Ca^<2+> and released Ach, whereas synaptic vesicles did not interact with non-pretreated membrane. However, Ca^<2+> were not required for the fusion itself. Arachidonic acid potentiated the fusogenic activity of membrane. In rat parotid acinar cells as endocrine tissue, the activation of PLA_2 was involved in a fusion of and a release of amylase from the secretary ranules with plasma membrane. These finding suggest that PLA_2 activity is important in the process of fusion in the regulated exocytosis.
In conclusion, it was clear that PLA_2 has a pivotal role for regulating the exocytosis involving neurotransmitter release and enzyme secretion. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tadayoshi TAKEUCHI: "Ettects of H-89, an inhibitor of protein kinase A, on the acetylcholine release from myenteric plexus of guinea pig ileum." Journal of Smooth Muscle Research. 31. 143-151 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahiro NAGAO: "Ca^<2+>-independent fusion of secretory granules with phospholipase A_2-treated plasma membranes in vitro." Biochemical Journal. 307. 563-569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideaki NISHIO: "Ca^<2+>-independent fusion of synaptic vesicles with phospholipase A_2-treated presynaptic membranes in vitro." Biochemical Journal. 318. 981-987 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko SAITOH: "Muscarinic autoinhibition and modulatory role of protein kinase C in acetylcholine release from the myenteric plexus of guinea-pig ileum." Japanese Journal of Pharmacology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tadayoshi TAKEUCHI,Yuko FUKUNAGA,Fumiaki HATA and Osamu YAGASAKI: "Effects of H-89, an inhibitor of protein kinase A,on the acetylcholine release from myenteric plexus of guinea pig ileum." Journal of Smooth Muscle Research. 31-4. 143-151 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahiro NAGAO,Taeko KUBO,Ritsuko FUJIMOTO,Hideaki NISHIO,Tadayoshi TAKEUCHI and Fumiaki HATA: "Ca^<2+>-independent fusion of secretory granules with phospholipase A_2-treated plasma membranes in vitro." Biochemical Journal. 307. 563-569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideaki NISHIO,Tadayoshi TAKEUCHI,Fumiaki HATA and Osamu YAGASAKI: "Ca^<2+>-independent fusion of synaptic vesicles with phospholipase A_2-treated presynaptic membranes in vitro." Biochemical Journal. 318. 981-987 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko SAITOH,Ritsuko FUJIMOTO,Toshiaki ISHII,Hideaki NISHIO,Tadayoshi TAKEUCHI and Fumiaki HATA: "Muscarinic autoinhibition and modulatory role of protein kinase C in acetylcholine release from the myenteric plexus of guinea-pig ileum." Japanese Journal of Pharmacology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tadayoshi TAKEUCHI: "Effects of H-89, on inhibitor of protein kinase A, on the aietylcholine release troin myenteric plexus of guinea pig ileum" Journal of Smooth Muscle Research. 31. 143-151 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahiro NAGAO: "Ca^<2+>-independent tusion of secretory granutes with phospholipase A_2-treated plasma membranes in vitro" Biochemical Journal. 307. 563-569 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hideaki NISHIO: "Ca^<2+>-independent tusion of synaptic vesicles with phospholipase A_2-treated presynaptic membranes in vitro" Biochemical Journal. 318. 981-987 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tadayoshi TAKEUCHI: "Effects of H-89,an inhibitor of protein kinase A,on the acetylcholine release from myenteric plexus of guinea pig ileum" Journal of Smooth Muscle Research. 31. 143-151 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahiro NAGAO: "Ca^<2+>-independent fusion of secretory granules with phospholipase A_2-treated plasma membranes in vitro" Biochemical Journal. 307. 563-569 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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