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Identification of receptor-binding site of the mouse hepatitis virus spike protein

Research Project

Project/Area Number 07660413
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNational Institute of Neuroscience, NCNP

Principal Investigator

TAGUCHI Fumihiro  National Institute of Neuroscience, NCNP,section chief, 神経研究所・モデル動物開発部, 室長 (30107429)

Co-Investigator(Kenkyū-buntansha) SUZUKI Hideka  National Institute of Neuroscience, NCNP,postdoc
鈴木 秀佳  国立精神, 神経センター・神経研究所, 流動研究員
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmouse hepatitis virus / coronavirus / spike protein / receptor-binding site
Research Abstract

We have previously shown that the N terminal region composed of 330 amino acids of MHV S protein (S1N330) is important for receptor-binding activity, since the S1N330 protein expressed by recombinant vaccina virus binds to the MHV-receptor protein prepared on the membrane paper by Western blot in a similar manner as whole S protein binds to the receptor protein. The aim of this experimetn is to identify the amino acids that participate in the receptor-binding activity in S1N330. We have found that 7MHV strains can use mmCGM1 as a functional receptor. In the S1N330 of these 7 MHV strains, we found 3 major consercative regions which include more than 10 consective amiano acids identical among these 7 MHV strains. We have introduced the mutations in some of amino asids in these regions and analyzed the receptor-binding activity of such mutant proteins. The mutant S1N330 protein with a mutation at amino acid 62 from the N terminus failed to bind to the receptor protein. The receptor binding og a mutant with mutations at 212,214, and 216 was remarkably reduced. These results imply that amino acids at 62,212,214 and 216 play important role for the receptor-binding activity of the MHV s protein. Furthermore, it is suggested that secondary or tertiary structure of SIN330 is necessary for receptor-binding activity, since the region containing amino acid 62 is located apart from a region including amino acids 212,214 and 216 in primary MHV s protein sturucture.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ohtsuka,N.,Y.K.Yamada,and F.Taguchi: "Difference in virus-binding activity of two distinct receptor proteins for mouse hepatitis virus" Journal of General Virology. 77. 1683-1692 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,H.,and F.Taguchi: "Analysis of the receptor-binding site of murine coronavirus spike protein" Journal of Virology. 70. 2632-2636 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada,Y.K.,M.Takimoto,M.Yabe,and F.Taguchi: "Aquired fusion activity of a murine coronavirus MHV-2 variant with mutation in the proteolytic cleavage site and the signal sequence of the S protein" Virology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田口文広: "マウス肝炎ウイルススパイク蛋白の構造と機能" ウイルス. 46. 109-117 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi, F., Kubo, H., Takahashi, H., and Suzuki, H.: "Localization of neurovirulence determinant for rats on the S1 subunit of murine coronavirus JHMV" Vorology. 208. 67-74 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi, F., Kubo, H., Suzuki, H., and Yamada, Y.: "Localization of neutralizing epitopes and receptor-binding site in murine coronavirus spik protein" Adv. Exp. Med. Biol.380. 359-365 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi, F., Suzuki, H., Takahashi, H., and Kubo, H.: "Neurovirulence forrats of the JHM variants escaped from neutralization with S1-specific monoclonal antibodies" Adv. Exp. Med. Biol.380. 185-187 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi, F.: "The S2 subunit of the murine coronavirus spike protein is not involved in teh receptor binding" J.Virol.69. 7260-7263 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, H., and Taguchi, F.: "Analysis of receptor-binding site of the murine coronavirus spike protein" J.Virol.70. 2632-2636 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtsuka, N., Yamada.Y.K., and Taguchi, F.: "Difference in virus-binding activity of two distinct receptor proteins for mouse hepatitis virus" J.Gen. Virol. 77. 1683-1692 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] yamada, Y.K., Takimoto, K., Yabe, K., and Taguchi, F.: "Acquired fusion activity of a murine coronavirus MHV-2 variant with mutations in the proteolytic cleavage site and the signal sequence of the S protein" Virology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi, F.: "Structure and biological functions of the spike protein of mouse hapatitis virus" Uirusu. 62. 109-117 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtsuka,N.,Y.K.Yamada,and F.Taguchi: "Difference in virus-binding activity of two distinct receptor proteins for mouse hepatitis virus" Journal of General Virology. 77. 1683-1692 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,H.,and F.Taguchi: "Analysis of the receptor-binding site of murine coronavirus spike protein" Journal of Virology. 70. 2632-2636 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamada,Y.K.,M.Takimoto,M.Yabe,and F.Taguchi: "Aquired fusion activity of a murine coronavirus MHV-2 variant with mutation in the proteolytic cleavage site and the signal sequence of the S protein" Virology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田口文広: "マウス肝炎ウイルススパイク蛋白の構造と機能" ウイルス. 46. 109-117 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohtsuka, N, Yamada, Y. K., & Taguchi, F: "Difference in virus binding activity of two distinct receptor proteins for mouse nepatitis virus" Journal of general Virology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki, H., and Taguchi, F: "Analysis of receptor-binding site of the murine coronavirus spike protein." Journal of Virology. 70(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Taguchi, F: "The Sa subunit of murine coronavirus spike protein is not involued in receptor binding" Journal of Virology. 69. 7260-7263 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Taguchi, F., et al.: "Neurovirulence for rats of the JHM variants escaped from neutralization with S, specific monoclonal antibodies." Advances of the Experimental medicine and Biology. 380. 185-187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Taguchi, F., et al.: "hocalization of neutralizing epitopes and receptor-binding site in murine eoronavirus spike protein" Advances of the Experimental medicine and Biology. 380. 359-365 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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