| Project/Area Number |
07660414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Faculty of Agriculture, Iwate University |
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Principal Investigator |
SHINAGAWA Kunihiro Faculty of Agriculture, Iwate University, Professor, 農学部, 教授 (60133906)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Norio Faculty of Agriculture, Iwate University, Associated professor, 農学部, 助教授 (40092308)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Staphylococcus aureus / Enterotoxin A / Synthetic peptide / Epitope / INF- / Bacillus cereus / HEp-2 vacuole factor / Emetic toxin / Suncus / 抗ペプチド抗体 / エビトープ / セレウス菌 / 嘔吐毒 / エンテロトキシン / 催吐活性 / 致死活性 |
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| Research Abstract |
The staphylococcal enterotoxins (SEs) are serologically classified in five types(A,B,C : C_1, C_2, C_3, D,and E). It was not yet fully understood by these studies to anaylyze the biologically and functionalyy active sites of SES.Attempts were made to investigate the antigenic determinants (epitopes) of SE-A.The results obtained were as follows.
1.Native SE-A molecule contains at least 3 different antigenic determinants (synthetic peptides A-1 : amino acid residues 1-20, A-5 : 81-100 and A-8 : 141-160).
2.Amino acid residue 161-180 on SE-A molecule is an essential region for production of murine INF-.
To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting type food poisoning, emetic activities of purified HEp-2 factor (Cereulid) to rhesus monkeys and suncus were determined. The results obtained were as follows.
1.The purified Cereulide (HEp-2 factor) is strongly suggested to be an emetic toxin itself.
2.The Cereulide is stable in the digestive tracts, induce emesis, and show lethal activity leading to be cellular damage.
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