Mechanism of Competitive Exclusion of Salmonella by Intestinal Microflora in Chickens
| Project/Area Number |
07660423
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
BABA Eiichiro Osaka Prefecture University, Faculty of Agriculturer Associate Professor, 農学部, 助教授 (70081594)
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| Co-Investigator(Kenkyū-buntansha) |
SASAI Kazumi Osaka Prefecture University, Faculty of Agriculturer, Assistant Professor, 農学部, 助手 (70211935)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | competitive exclusion / adhesion / intestinal flora / Salmonella / chicken / continuous flow culture / yeast / mannose residue / 接着剤 / ニワトリ |
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| Research Abstract |
Intestinal microflora has the ability to exclude newly introduced pathogens. However, the precise mechanism responsible for the competitive exclusion by bacterial flora is unknown. We conducted two experimental works for solving this problem, especialy on competition of atachment teritory by bacteria. 1) Colonizing competition between Salmonella typhimurium and bacterial combination was analyzed using anaerobic continuous flow culture system. Growth of S.typhimurium was suppressed when inoculated into the flow culture of pre-colonized bacterial combination with Escherichia coli, Lactobacillus spp.and Bifidobacterium thermophilum. However, S.typhimurium was not suppressed by the bacterial combination when inoculated after S.typhimurium was already colonized. Bacterial adhesion observed under scanning electron microscope offered the visual evidence to support the important tool of adhesion in competitive exclusion. 2) An anaerobic continuous flow (CF) culture system was utilized to isola
… More
te from the cecal contents of adult chickens some indigenous cecal bacteria with high ability of attachment. The protective effects of the combination of isolated bacteria on S.typhimurium colonization was evaluated in CF culture and layr chicks. Dietary dry yeast was also examined for protective effect against salmonella colonization in chicks inoculared with the bacterial combination. In the CF culture system, S.typhimurium population was suppressed when pre-colonized with the bacterial combination. Compared with controls, the mean number of S.typhimurium in the cecal contents of the chicks given with the bacterial combination was significantly (P<0.05) low 3 and 5 days after Salmonella inoculation. But the effect of the bacterial combination was not clear with low number of Salmonella challenge. Compared with controls, the mean number of S.typhimurium in the cecal contents of chicks given with the bacterial combination and dry yeast was significantly (P<0.05) low 1 and 3 days after Salmonella inoculation. Similarly, the depressive effects of the bacterial combination and dry yeast were shown with the low number exposure of Salmonella. Less
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Report
(3 results)
Research Products
(6 results)
