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Characterization of leukocyte functions from cattle with leukocyte adhesion deficiency (BLAD) and prevalence of BLAD-carrier in Holstein cattle in Japan

Research Project

Project/Area Number 07660426
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionRAKUNO GAKUEN UNIVERSITY,FACULTY OF VETERINARY MEDICINE

Principal Investigator

NAGAHATA Hajime  RAKUNO GAKUEN UNIVERSITY,FACULTY OF VETERINARY MEDICINE,ASSOC.PROFESSOR, 獣医学部, 助教授 (10133571)

Co-Investigator(Kenkyū-buntansha) GARY J. Koci  米国オハイオ州立大学, 獣医学部, 教授
TAMOTO Kouichi  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO,FACULTY OF PHARMACEUTICAL SCIENCE,ASSOC.P, 薬学部, 助教授 (50088861)
TANIYAMA Hiroyuki  RAKUNO GAKUEN UNIVERSITY,FACULTY OF VETERINARY MEDICINE,ASSOC.PROFESSOR, 獣医学部, 助教授 (90133800)
KOCIBA Gary J  THE OHIO STATE UNIVERSITY,COLLEGE OF VETERINARY MEDICINE,PROFESSOR
GARY J Kocib  米国オハイオ洲立大学, 獣医学部, 教授
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsBLAD / CD18 / bovine immunodeficiency / leukocyte functions / BLAD-carrier / disease control / 好中球機能 / DNA-PCR / キャリャ-率 / 遺伝子頻度 / CD18 / 免疫組織検索 / Fcリセプター / BLAD-キャリャ-
Research Abstract

The above-entitled study was performed and the results were summarized as follows :
Severe bone resorption, osteolysis and severe progressive periodontitis in submandibula due to dysfunction of leukocytes in heifers with BLAD were demonstrated by radiographic examination. Immunohistochemical analysis of lymph nodes using anti-CD18 antibody demonstrated that CD18-positive cells were not found on those from the BLAD-heifer.
Fc receptor expression, cytoplasmic Ca^<2+> signaling, chemiluminescent (CL) response, and electron spin resonance (ESR) combined with spin trapping of blood mononuclear phagocytes from control heifers and a heifer with BLAD were evaluated to elucidate the relationships between complement receptor type 3 (CR3) and Fc receptor expression and their functional responses. Fc receptors on mononuclear phagocytes from the heifer with BLAD were enhanced, and their cytoplasmic Ca^<2+> singnaling, CL responses, and ESR-spin trapping when stimulated with aggregated IgG (Agg-IgG) o … More psonized zymosan (OPZ) appeared to be associated with enhanced Fc receptors.
The adhesiveness of control and CD18-deficient bovine neutrophils on culture plates precoated with collagen I,collagen IV,fibronectin and laminin was measured to evaluate the possible factors for adherence to extracellular matrices. The adhesiveness activities of CD18-deficient neutrophils to collagen I,collagen IV and fibronectin were significantly diminished ; however, similar adhesion to laminin was observed in CD18-deficient neutrophils and control neutrophils.
Biosynthesis of CD11/CD18 in bovine leukocytes, intracellular Ca^<2+> signaling, CL responses and membrane fluidity of neutrophils from control heifers and a BLAD-heifer were measured. The synthesis of CD11/CD18 complex was clearly detected in leukocytes from a normal heifer, but not in a BLAD-heifer. The ttransient phase of increased Ca^<2+> was clearly detected in neutrophils from a BLAD-heifer stimulated with OPZ,Agg-IgG,whereas the sustained phase was deficient or significantly decreased compared with controls. No differences in membrane fluidity were detected between neutrophils derived from control and BLAD-cattle.
Blood samples from 796 Holstein dairy cows in 20 herds from 6 districts in Japan were examined to determine whether they were BLAD-free, BLAD carriers, or BLAD-affected by use of DNA-PCR analysis, BLAD-carrier rates ranged from 0 to 23.5% and the mean BLAD-carrier rate was 8.1% (70/796). Approximately 35% of the dairy herds had a prevalence of more than 10% of BLAD-carriers. The rate of the occurrence of BLAD in Japanese Holstein dairy cattle was estimated to be 0.16% at birth. A program for the eradication of the gene encoding impaired CD18 for BLAD in Holstein cattle is slowly being enacted in Japan.
These results demonstrate that pathophysiological changes of BLAD are closely associated with the deficiency of CD18 expression on BLAD-leukocytes. This study provide a valuable information for the control of BLAD in Japanese Holstein cattle. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Nagahata,H.,et al.: "Radiographic and immunohistochemical analysis of leulkocyte adhesion deficiency in Holstein heifers." Canadian Journal of Veterinary Research. 59. 316-318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 永幡 肇・森田光夫: "牛白血球粘着不全症(BLAD)とその遺伝子診断" 動物遺伝研究会誌(J.Anim.Genet.). 23. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagahata,H.,et al.: "Functional characteristics of enhanced Fc reuptor expression of β_2-integrin deficient bovire monocytes." Microbiology and Immunology. 40. 389-395 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagahata,H.,et al.: "Adhesiveness for exfracellular matrices and lysosomal enzyme release from normal and BLAD-nentrophils." Microbiology and Immunology. 40. 783-786 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagahata,H.,et al.: "Biosynthesis of β_2 integrin, calcium signalling and functional respences of borine nectrophils." Research in Veterinary Science. 61. 95-101 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagahata,H.,et al.: "Preralence and allele freguency estimation of bovine LAD in Holseins in Japan." Journal of Veterinary Medical Science. 59(4). in press (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 桑原・郷司・稲波・樋口・永幡: "白血球粘着蛋白質欠損症ホルスタイン牛(BLAD)好中球を用いた活性酸素生成・情報伝達機構の研究" 編集 内海英雄,吉川敏一(日本医学館)磁気共鳴と医学(8刊), 5(109-113) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Radiographic and immunohistochemical analysis of leukocyte adhesion deficiency in Holstein heifers." Can.J.Vet.Res.59. 316-318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Functional characteristics of enhanced Fc receptor expression of B_2 integrin-deficient bovine mononuclear phagocytes." Microbiol.Immunol. 40. 389-395 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Adhesiveness for extracellular matrices and lysosomal enzyme release from normal and B_2 integrin-deficient bovine neutrophils." Microbiol.Immunol. 40. 783-786 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Neutrophil and mononuclear phagocyte functions from cattle with leucocyte adhesion deficiency (BLAD) and prevalence of BLAD-carrier in Holstein cattle in Japan." Proceedings of XIX World Buiatrics Congress. 2. 489-492 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Biosynthesis of B_2 integrin, intracellular calcium signalling and functional responses of normal and CD18-deficient bovine neutrophils." Res.Vet.Sci.61. 95-101 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nagahata: "Prevalence and allele frequency estimation of bovine leukocyte adhesion deficiency (BLAD) in Holstein-Friesian cattle in Japan." J.Vet.Med.Sci.59 (4) : (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagahata H,et al.: "Adhesiveness for extracellular matrices and lysosomal onzyme release from normal and B_2-integrin deficientborine neutnphils" Microbiology and Immunology. 40. 783-786 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahata,H,et al.: "Biosynthesis of B_2 integrin,intracellular Ca^<2+> signalling and functional responses of normal and CD18-deficient borine neutrophils." Research in Veterinary Science. 61. 95-101 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahata,H,et al.: "Neutrophil and mononuclear phagocyte functions from cattle with leucocyte adhesion deficiency (BLAD) and prevalence of …." Proceedings of XIX World Buiatrics Congress. 2. 489-492 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahata,H,et al.: "Prevalence and allele frequency estimation of bovine lenkocyte adhesion deficiency (BLAD) in cattle in Japan." Journal of Veterinary Medical Science. 59(4)(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahata et al.: "Radiographic and immunohistochemical analysis of leukocyte adhesion deficiency in Holstein neifers." Canadian Journal of Veterinary Research. 59. 316-318 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagahata et al.: "Intracellular calcium sigaling and funetional responses of β2 integrin deficient borine neutrophils." Research in Veterinary Science. (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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