| Project/Area Number |
07660428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Nihon University |
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Principal Investigator |
FUJIWARA Kousaku Nihon Univ.School of Bioresource Sciences, Professor, 生物資源科学部, 教授 (60012689)
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| Co-Investigator(Kenkyū-buntansha) |
MORITOMO Tadaaki Nihon Univ.School of Bioresource Sciences, Lecturer, 生物資源科学部, 講師 (20239677)
TSUKISE Azuma Nihon Univ.School of Bioresource Sciences, Professor, 生物資源科学部, 教授 (20059752)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | cholesterol feeding / cirrhosis / fat liver / Ito cell / Mongolian gerbil / コレステロール / 偽小葉 / PCNA |
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| Research Abstract |
Pathogenesis of cirrhotic liver lesions, in special reference to the activity of the Ito cells, was studied in Mongolian gerbils fed 0.2 to 1 % cholesterol containing diet. Shortly after starting the choresterol feeding, serum cholesterol levels were remarkably elevated, attaining a maximum level of 1,000 mg/dl in the 0.5 or 1 % cholesterol group. The liver of the cholesterol-fed animals was markedly enlarged and white-graysh in color showing a multiple nodular pattern. Histopathologically, hepatocytes were swollen with intracellular accumulations of cholesterol, resulting in the sinusoid narrowing. After longer period of cholesterol feeding, severe fat degeneration and necrosis were produced in hepatocytes of the zone 3, where the Ito cells were accumulated being positive for alpha-smooth muscle actin (alphaSMA) and forming intercellular fibrous networks. With the continuation of cholesterol feeding, the Ito cells were increased in number showing more intense production of argiophilic as well as collagenous fibers, which were finally connected with the fibers elongated from the Glisson's sheath. In 0.5 and 1 % cholesterol group atypical hepatocytes nodularly appeared in the zones 1 and 2 and were surrounded later by a denser fibrous band. In thus formed cirrhotic lesions the Ito cells had longer processes and seemed to liberate reticular and collagen fibers into the Disse spaces.
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