| Project/Area Number |
07660429
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Nippon Veterinary and Animal Science University |
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Principal Investigator |
HATAI Kishio Nippon Veterinary and Animal Science University, Faculty of Vaterinary and Animal Science, Professor, 獣医畜産学部, 教授 (30164841)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Lagenidiales / greasyback shrimp / kuruma prawn / crustaceans / fungal diseases / Haliphthoros / Halocrusticida / Haliphthoros milfordensis / Haliphthoros hiroshimaensis |
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| Research Abstract |
A strain isolated from zoea larvae of kuruma prawn (Penaeus japonicus) and two strains isolate from a egg and a mysis larvae of greasyback shrimp (Metapenaeus ensis) were examined detailed to make an identification. The isolation was made by inoculating whole of affected organism onto PYGS agar and incubaing at 20ﾟC.For inhibition of bacterial growth, the addition of 50mug/ml each of streptomycin sulphate and ampicillin to the medium was required. For morphological observation, the strains were incubated in PYGS broth at 25ﾟC for three days to grow mycelia. The mycelia were then washed twice with sterilized artificial seawater and incubated at 25ﾟC to induce zoospore formation. As the results, all isolates were classified into the fungi of the genus Haliphthoros from the characteristic of the fragment formations. And two isolates and the rest were identified as H.milfordensis and a new species of the genus. H.hirosimaensis, respectively.
Each one strain isolated from kuruma prawn and greasyback shrimp was made to identify by same method. They were classified into the genus Halocrusticida from the characteristic of gemmae production and so on. And each isolate from kuruma prawn and greasyback shrimp was identifies as H.panulirata and H.parasitica, respectively.
Additionally, some physiological characteristics of the isolates were examined.
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