• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

THE MORPHOLOGICAL ANALYSIS OF THE PERITONEUM IN DEFENCE SYSTEM

Research Project

Project/Area Number 07670002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

SASAKI Katsunori  YAMAGATA UNIVERSITY,SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (30170666)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPERITONEUM / PERITONITIS / CELLULAR ADHESION MOLECULES / immunoSEM
Research Abstract

To establish the peritoneum as defense organ, interaction between the peritoneal mesothelial cells and immuneassociated cells were analyzed morphologically through expression of cellular adhesion molecules with immunoSEM.ICAM-1 (intercellular adhesion molecule-1) is an important molecule of IgG superfamily associated with leukocyte migration through stick adhesion. This molecule was selectively expressed on the microvilli of the peritoneum and was upregulated by LPS (Lipopolysaccharide). The peak was 24 hrs after stimulation. ICAM-1 was suggested to work in both normal and inflammatory conditions.
In the experimental peritonitis, a large number of macrophages appeared on the peritoneum. They had LFA-1 (leukocyte function-associated antigen 1) and Mac-1, which are ligands of ICAM-1, on the cell surface. Mac-1 was restricted to the microvilli of the macrophages in activated condition. The microvilli of the peritoneum expressed ICAM-1 moderately. It was confirmed that these abhesions was necessary for iinteraction between the peritoneum and leukocytes.
The peritoneum expressed VCAM-1 in addition to ICAM-1 after LPS stimulation. Other adhesions (ICAM-2, ELAM-1, PECAM-1, CD105) were not detected. VCAM-1 was restricted to microvilli as well as ICAM-1. After both expression, s leukocytes fixed to the peritoneum Double immunostaining showed localizations of ICAM-1 and VCAM-1 were near to each other.
These results showed that (1) Microvilli with ICAM-1 is a road for leukocyte migration in the normal condition and (2) in inflammation, ICAM-1 and VCAM-1 cooperate to fix leukocytes to the peritoneum and the peritoneum works as defense organ through interaction of ICAM-1 and VCAM-1 with LFA-1, Mac-1 and VLA-4.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sasaki, K.et al.: "Ultrastructural localization of the intercellular adhesion molecule(ICAM-1)on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, K.et al.: "Early expression of intercelluar adhesion molecule-1 in the corneal endothelium stimulated by endotoxin:An immuno-scanning electron microscopical analysis." Jpn.J.Opthalmol.40. 12-17 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, K.et al.: "Distribution of intercellular adhesion molecule-1 on leukocytes and corneal endothelium after endotoxin stimulation in rats." Int.Opthalmol.19. 303-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shao, F.et al.: "Effect of basic fibroblast growth factor on angiogenesis in rabbit flexor digitorum profundus tendons lacerated and repaired in the avascular area." Hand.Surg.2. 35-43 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo, H.et al.: "Three-dimensional and ultrastructural ICAM-1 distribution in the choroid plexus,arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection" Brain Res.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki, K.et al.: "Three-dimensional distribution of intercellular adhesion molecule-1(ICAM-1)on lymphocytes in the HEV analyzed by backscatter electron(BSE)imaging." Acta Anat.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki, K.et al.: "Ultrastructural localization of the intercellular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamagushi, K.et al.: "Early expression of intercellular adhesion molecule-1 in the corneal endothelium stimulated by endotoxin : An immuno-scanning electron microscopic analysis." Jpn.J.Opthalmol.40. 12-17 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, K.et al.: "Distribution of intercellular adhesion molecule-1 on lleukocytes and corneal endothelium after endotoxin stimulation in rats." Int.Opthalmol.19. 303-306 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shao, F.et al.: "Effect of basic fibroblast growth factor on angiogenesis in rabbit flexor digitorum profundus tendons lacerated and repaired in the vascular area." Hand.Surg.2. 35-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo, H.et al.: "Three-dimensional and ultrastructural ICAM-1 distribution in the choroid plexus, arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection." Brain Res.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki, K., et al.: "Three-dimensional distribution of intercellualr adhesion molecule-1 (ICAM-1) on lymphocytes in the HEV analyzed by backscatter electron (BSE) imaging." Acta.Anat.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki,K. et al.: "Ultrastructural localization of the intercellular adhesion molecule(ICAM-1)on the cell surface of high endothelial venules in lymh nodes." Anat.Rec.244. 105-111 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,K., et al.: "Early expression of intercelluaradhesion molecule-1 in the corneal endothelium stiulated by endotoxin:An immuno-scanning electron microscopical analysis." Jpn:J.Opthalmol.40. 12-17 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,K., et al.: "Distribution of intercellular adhesion molecule-1 on leukocytes and corneal endothelium after endotoxin stimulation in rats." Int.Opthalmol.19. 303-306 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shao,F. et al.: "Effect of basic fibroblast growth factor on angiogenesis in rabbit flexor digitorum profundus tendons lacerated and repaired in the avascular area." Hand.Surg.2. 35-43 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Endo,H. et al.: "Three-dimensional and ultrastructural ICAM-1 distribution in the choroid plexus,arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection" Brain Res.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Sasaki,K. et al.: "Three-dimensional distribution of intercellular adhesion molecule-1(ICAM-1) on lymphocytes in the HEV analyzed by backscatter electron(BSE) imaging." Acta Anat.(revised).

    • Related Report
      1997 Annual Research Report
  • [Publications] Sasaki,K.et al.: "Ultrastructural localization of the intercellular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,K.et al.: "Early expression of intercellular adhesion molecule-1 in the corneal endothelium stimulated by endotoxin : An immuno-scanning electron microscopical analysis." Jpn.J.Opthalmol.40. 12-17 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,K.et al.: "Distribution of intercellular adhesion molecule-1 on leukocytes and corneal endo thelium after endotoxin stimulation in rats." Int.Opthalmol.19. 303-306 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,K.,et al.: "Ultrastructural localization of the intercelluar adhesion molecule(ICAM-1)on the cell surface of high emdothelial venules in lympy nodes." Anat.Rec.244. 105-111 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamaguchi,K.,et al.: "Early expression of intercelluar adhesion molecule-1 in the corneal endothelium stimulated by endotoxin:AN immuno-scanning electron microscopical analysis." Jpn.J.Ophthalmol.40(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamaguchi,K.,et al.: "Distribution of intercellular adhesion molecule-1 on leukocytes and corneal endothelium after endotoxin stimulation in tats." Int.Ophthalmol.(発表予定). (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi