• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of three-dimensional gel of extracellular matrices including type VI collagen and its role for immune reaction

Research Project

Project/Area Number 07670009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya University

Principal Investigator

KOBAYASHI Miya  Nagoya University School of Medicine, Department of Anatomy, Associate Professor, 医学部, 助教授 (70002178)

Co-Investigator(Kenkyū-buntansha) 星野 洸  名古屋大学, 医学部, 教授 (40000913)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsImmune reaction / Langerhans cell / Extracellular matrix / Type VI collagen / Proteoglycan / Skin / Collagen fibril / Electron microscopy / ATP / マクロファージ / 三次元ゲル / 免疫電顕法
Research Abstract

Epidermal Langerhans cells are now recognized as designed to present antigenic signals to Tlymphocytes and trigger an immunologic response. The cells also exist in the stratified squamous mucosal epithelium of mouse forestomach and tongue. Langerhans cells reside in the epidermis, and migrate into dermis, finally to the draining lymph nodes, where they present antigenic signals to T lymphocytes. For the search of interaction between extracellular matrices and immune cells, it is useful to study migration pathway of Langerhans cells in the dermis.
Among many extracellular matrices, type VI collagen is a macromolecular component of extracellular matrices. They were widely distributed in connective tissues. Electron microscopic examinations and analysis of the amino acid sequences revealed that type VI collagen may work as an anchor in cell-matrix or matrix-matrix interaction.
We investigated the effect of fibril-forming collagens or other extracellular matrices on the adhesion of macrophages. Adhesion of macrophages to plastic or fibronectin was associated with the induction of tyrosine phosphorylation. In contrast, the induction of protein tryosine phosphorylation was markedly inhibited on type I collagen. Type I collagen is a non-adhesive extracellular matrix causing cell migration on immuno reactions.
The surface structure of D-periodic striated collagen fibrils of mouse tail tendon was examined by atomic force microscopy (AFM). Native collagen fibrils were spread on the cover glass and observed with AFM without any treatment. The band depth was estimated. A quantitative evaluation of collagen structural features in the nanometer scale is made possible by AFM.This microscopy is also useful to study three-dimensional structure of the gel composed of extracellular matrices.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Mitsudo K: "Electron-microscopic and immunohistochemical studies of Langerhans cells and Thy-1-positive cells in mouse tongue epithelium subjected to local hyperthermia" Archives of Oral Biology. 40. 533-538 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Senga K: "Type VI collagen in mouse masseter tendon, from osseous attachment to myotendinous junction" Anatomical Record. 243. 294-302 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto T: "Type VI collagen periodic fibrils in the synovium of the mouse temporomandibular joint" Archives of Oral Biology. 40. 975-978 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小林身哉: "VI型コラーゲンの機能に関する実験的考察" 解剖学雑誌. 70. 298-306 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura S: "Immunoelectron microscopic Iocalization of decorin in aged human corneal and scleral stroma" Journal of Electron Microscopy. 44. 445-449 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe M: "Langerhans cels in mice with severe combined immunodeficiency" Archives of Histology and Cytology. 59. 347-355 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iida K,Kobayashi M,Kobayashi K,Saga S,Hoshino T,Matsuyama M.: "Experimental in vivo and in vitro formation of type VI collagen periodic fibrils in chorionic villi of human" J Electron Microsc. 43-6. 367-372 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura M,Kobayashi M,Hirano K,Kobayashi K,Hoshino T,Awaya S.: "Glycosaminoglycan and collagen fibrillar interactions in the mouse corneal stroma." Matrix Biology. 14. 283-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Niwa J,Kobayashi M,Koyama Y,Kotani H,Mitsudo K,Hoshino T.: "Langerhans cells closely associated with Thy-1-positive dendritic cells in the stratified squamous mucosal epithelium of mice." Arch Histol Cytol. 57-3. 259-266 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasue K,Kobayashi M,Hattori H,Teramoto T,Senga K,Mizutani H,Ueda M,Kaneda T,Hoshino T.: "An ultrastructural study of extracellular fibrillar components of developing mouse mandibular condyle with special reference to type VI collagen." Arch Oral Biol. 39-8. 689-694 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitsudo K,Kobayashi M,Tohnai I,Ueda M,Kotani H,Hoshino T.: "Electron-microscopic and immunohistochemical studies of Langerhans cells and Thy-1-positive cells in mouse tongue epithelium subjected to local hyperthermia." Arch Oral Biol. 40-6. 533-538 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Senga K,Kobayashi M,Hattori H,Yaseu K,Mizutani H,Ueda M,Hoshino T.: "Type VI collagen in mouse masseter tendon, from osseous attachment to myotendinous junction." Anat Rec. 243-2. 294-302 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto T,Kobayashi M,Mizuno I,Yasue Y,Mizutani H,Ueda M,Hoshino T.: "Type VI collagen periodic fibrils in the synovium of the mouse temporomandibular joint." Arch Oral Biol. 40-10. 975-978 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura S,Kobayashi M,Nakamura M,Hirano K,Awaya S,Hoshino T.: "Immunoelectron microscopic localization of decorin in aged human corneal and scleral stroma." J Electron Microsc. 44-6. 445-449 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe M,Kobayashi M,Iida K,Ozek M,Doi S and Hoshino T.: "Langerhans cells in mice with severe combined immunodeficiency" Arch Histol Cytol. 59-4. 347-355 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitsudo K: "Electron-microscopic and immunohistochemical studies of Langerhans cells and Thy-1-positive cells in mouse tongue epithelium subjected to local hyperthermia" Archives of Oral Biology. 40・6. 533-538 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Senga K: "Type VI collagen in mouse masseter tendon,from osseous attachment to myotendinous junction" Anatomical Record. 243・2. 294-302 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto T: "Type VI collagen periodic fibrils in the synovium of the mouse temporomandibular joint" Archives of Oral Biology. 40・10. 975-978 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小林身哉: "VI型コラーゲンの機能に関する実験的考察" 解剖学雑誌. 70・4. 298-306 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kimura S: "Immunoelectron microscopic localization of decorin in aged human corneal and scleral stroma" Journal of Electron Microscopy. 44・6. 445-449 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watanabe M: "Langerhans cells in mice with severe combined immunodeficiency" Archives of Histology and Cytology. 59・4. 347-355 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Senga,K,Kobayashi M et al.: "Type VI Collagen in mouse masseter tendon,from osseous attachment to myotendinous junction" Anatomical Record. 243. 294-302 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Teramoto T,Kobayashi M et al.: "Type VI collagen periodic fibrils in the synovium of the mouse temporomandibular joint" Archives of Oral Biology. 40. 975-978 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小谷久也、小林身哉ら: "粘膜上皮の成熟とランゲルハンス細胞の動態" 日本口腔科学会雑誌. 44. 77-82 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小林身哉: "VI型コラーゲンの機能に関する実験的考察" 解剖学雑誌. 70. 298-306 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura S,Kobayashi M et al.: "Immunoelectron microscopic localization of decorin in aged human corneal and scleral stroma." Journal of Electron Microscopy. 44. 445-449 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 安江一紀、小林身哉ら: "マウス下顎頭線維層における弾性線維システムの局在" 歯科基礎医学会雑誌. 38(in press). (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi