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Apoptosis and epithelium-mesenchyme interaction during mammalian morphogenesis

Research Project

Project/Area Number 07670012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MORI Chisato  Kyoto Univ.Fac.of Med.Associate Professor, 医学研究科, 助教授 (90174375)

Co-Investigator(Kenkyū-buntansha) IRIE Hidekazu  Kyoto Univ.Fac.of Med., 医学研究科, 日本学術振興会特別研
SHIOTA Kohei  Kyoto Univ.Fac.of Med.Professor, 医学研究科, 教授 (80109529)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsprogramd cell death / morphogenesis / apoptosis / palate / limb / spermatogenesis / gene / abnormal development
Research Abstract

Apoptosis is a common event during mammalian morphogenesis, which is morphologically known as programd cell death. The cell death in interdigital spaces of mammalian developing limb and the disappearance of the midline epithelial seam during the fusion process of the mammalian secondary palate are classical examples of morphogenetic programd cell death. For the formating of various structure during development, apoptosis has been considered to play a key role by elimination unnecessary cells to achieve complicated histogenesis and organogenesis. Our recent studies showed that apoptosis in the limb and palate development plays more aggressive roles in addition to the elimination of unnecessaery cells. In mammalian limb development, apoptosis may play a role in preventing the formation of extra digits and reducing the size of the first digit as well as in the digit separation and joint formation (Mori et al., 1995 ; Mori, 1995). In the process of palate fusion, apoptotic cell death may be required for the midline epithelial fusion of opposing palatal shelves (Mori et al., 1994). In addition, we examined the cause of limb malformations induced by BrdU in fetal mice and found that the altered expression of several genes including apoptosis-related genes, Hox genes and growth factor genes at the anterior region of the hindlimb bud in the formation of preaxial polydactyly and triphalangism of the first digit in BrdU-treated mouse fetuses. Moreover, our analysis of apoptosis in the testes of some testis-specific gene deficient mice showed that an additional increased level of apoptosis during the first wave of spermatogenesis, in pachytene spematocytes (Mori et al., 1997). further studies will be necessary to define clearly the significance of apoptosis during mammalian morphogenesis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Mori,C. et al.: "Cytochemical identification of programmed cell death in the fusing fetal mouse palate by specific labeling of DNA fragmentaion." Anat Embryol. 190. 21-28 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori,C. et al.: "Programmed cell death in the interdigital tissue of the fetal mouse limb is apoptosis with DNA fragmentation." Anat Rec. 242. 103-110 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori,C.: "Significance of programmed cell death (apoptosis) in mammalian limb and palate development." Acta Anat Nippon. 70. 503-508 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori,C. et al.: "Testis-specific expression of mRNAs for a unique human type I hexokinase lacking the porin-binding domain." Mol Reprod Dev. 44. 14-22 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori,C. et al.: "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp70-2 knockout mice." Dev Dyn. 208. 125-136 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori, C., Nakamura, N., Okamoto, Y., Osawa, M., and Shiota, K.: "Cytochemical identification of programd cell death in the fusing fetal mouse palate by specific labelling of DNA fragmentation." Anatomy nad Embryolgy. 190. 21-28 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori, C., Nakamura, N., Kimura, S., Irie, H., Takigawa, T., and Shiota, K.: "Ptogramd Cell Death in the Interdigital Tissue of the Fetal Mouse Limb is Apoptosis With DNA Fragmentation." The Anatomical Record. 242. 103-110 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shiota, K.: "Morphogenesis and Apoptosis (in Japanese)" Nissanpu kantoregou-bukai kaihou. 32. 63-67 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori, C.: "Significance of Programd Cell Death (Apoptosis) in Mammalian Limb and Palate Development" Acta Anatomica Nipponica. 70. 503-508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori, C., Nakamura, N., Dix, DJ., Fujioka, M., Nakagawa, S., Shiota, K., and Eddy, EM.: "Morphogical Analysis of Germ Cell Apoptosis During Postnatal Testis Development in Normal and Hsp 70-2 Knockout Mice." Developmental Dynamics. 208. 125-136 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori,C.et al.: "Cytochemical identification of programmed cell death in the fusing fetal mouse palate by specific labeling of DNA fragmentaion." Anat Embryol. 190. 21-28 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,C.et al.: "Programmed cell death in the interdigital tissue of the fetal mouse limb is apoptosis with DNA fragmentation." Anat Rec. 242. 103-110 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,C.: "Significance of programmed cell death (apoptosis) in mammalian limb and palate development." Acta Anat Nippon. 70. 503-508 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,C.et al.: "Testis-specific expression of mRNAs for a unique human type 1 hexokinase lacking the porin-binding domain." Mol Reprod Dev. 44. 14-22 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,C.et al.: "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp70-2 knockout mice." Dev Dyn. 208. 125-136 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森 千里: "精子形成過程に特異的な遺伝子発現" 細胞. 27. 422-427 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori, C. et al.: "Programmed cell death in the interdigital tissue of the fetal mouse is apoptsis with DNA fragmentation" Anatomical Record. 242. 103-110 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mori, C.: "Significance of programmed cell death(apoptsis) in mammalian limb and palate development" Acta Anat Nippon. 印刷中 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mori, C. et al.: "Testis-Specific expression of mRNAs for a unique human type 1 hexokinase lacking the Porin-Binding domain" Mol. Reprod. Develop.印刷中 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 塩田 浩平: "器官形成とアポトーシス" 日産婦関東連合地方部会会報. 32. 63-67 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uehara, Y. et al.: "Placental defect and embryonic lethality in mice lacking hepatocyte growth factor" Nature. 373. 702-705 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oka, C. et al.: "Disruption of the mouse RBK-Jk gene results in early embryonic death" Development. 121. 3291-3301 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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