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Immunolocalizations of ET-1, ET receptors and CGRP in endothelial cells

Research Project

Project/Area Number 07670044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

FUJIMOTO Sunao  Univ. Occup. Environ. Health, Sch. Med., Dept. Anat., Professor, 医学部, 教授 (80080547)

Co-Investigator(Kenkyū-buntansha) DOI Yoshiaki  Univ. Occup. Environ. Health, Sch. Med., Dept. Anat., Associate Professor, 医学部, 助教授 (30258602)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsendothlin-1 / CGRP / Weibel-Palade body / endothelin receptors / enous sinus of dura mater vascular / carotid body artery / immunocytochemistry / endothelial cell / エンドセリン-1 / CGRP / ワイベル・パラ-デ顆粒 / 血管内皮細胞 / 脳硬静脈洞 / ビック・エンドセリン
Research Abstract

Vascular endothelial cells play the improtant roles in regulation of the local blood flow by producing some vasoactive peptides such as endothelin (ET) -1 and calcitonin gene-related peptide (CGRP), and by releasing them into both vascular lumen and subendothelial layr. Our immunoelectron microscopy revealed that ET-1 and CGRP are synthesized and segregated in the rough endoplasmic reticulum-Golgi system, stored in part in Weibel-Palade (WP) bodies in endothelial cells of the transverse venous sinus of the rat dura mater and the artery of the rat carotid body. In addition, we confirmed that both peptides are extracellularly released from the WP bodies into both vascular lumen and subendothelial layr by exocytosis in a manner of regulated pathway.
It is widely accepted that EP-1 induces vasoconstriction mediated from ETA receptors on the sarcolemma of medial muscle cells in a manner of paracrine, and vasodilatation mediated from ETB receptors on the plasmalemma of endothelial cells in a manner of autocrine. The present immunocytochemisty showed that immunoreactivities of the ETB receptors are localized to the plasmalemma of endothelial cells of the transverse venous sinus and the artery in the carotid body, and that those of ETA receptors are localized to the sarcolemma of medial muscle cells of the transverse venous sinus. In the arterial system of the carotid body, we could not detect out immunoreactions of the ETA receptors. These findings suggest that ET-1 regulates the blood flow in the transverse sinus by inducing both vasocontraction mediated from ETA receptors and vasodilatation mediated from ETB receptors, and that ET-1 preserves an abundance of the blood flow through the carotid body by evoking the vasodilatation mediated from ETB receptors.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yoshiaki Doi: "Histamine release from Weibel-Palade bodies of toad aortas induced by endothelin-1 and sarafotoxin" Anatomical Record. 242. 374-382 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kunshige Hamasaki: "The contraction of small arteries in the perimetrium by presurgical medication with the luteinizing hormone-releasing hormone agonist to patients with leiomyomas : An electron and immunoelectron microscopy" Fukuoka Acta Medica. 86. 389-397 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yosuke Kanazawa: "The roles of the marginal veins in the differentiation of the rat hind limb bud : an immunocytochemical study" Archives of Histology and Cytology. 59. 317-330 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Doi,Y.,Ozaka,T.,Fukushige,H.,Furukawa,H.,Yoshizuka,M.and Fujimoto,S.: "Increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta" Virchows Archiv. 428. 367-373 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ozaka,T.,Doi,T.,Kayashima,K.and Fujimoto,S.: "Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the carotid body" Anatomical Record. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Doi, Y., Ozaka, T., Katsuki, M., Fukushige, H., Toyama, E., Kanazawa, Y., Arashidani, K.and Fujimoto, S.: "Histamine release from Weibel-Palade bodies of toad aortas induced by endothelin-1 and sarafotoxin" Anatomical Record. 242. 374-382 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamasaki, K., Doi Y., Sakamoto, Y., Kashimura, M.and Fujimoto, S.: "The contraction of small arteries in the perimetrium by presurgical medication with the luteinizing hormone-releasing hormone agonist to patients with leiomyomas : An electron and immunoelectron microscopy" Fukuoka Acta Medica. 86. 389-397 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Doi, Y., Ozaka, T., Fukushige, H., Furukawa, H., Yoshizuka, M.and Fujimoto, S.: "The increase in number of Weibel-palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta" Virchows Archiv. 428. 367-373 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kanazawa, Y., Doi Y., Furukawa, H., Kayashima, K.and Fujimoto, S.: "The roles of the marginal veins in the differentiation of the rat hind limb bud : an immunocytochemical study" Archives of Histology and Cytology. 59. 317-330 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ozaka, T., Doi Y., Kayashima, K.and Fujimoto, S.: "Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body" Anatomical Record. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiaki Doi: "Histamine release from Weibel-Palade bodies of toad aortas induced by endothelin-1 and sarafotoxin" Anatomical Record. 242. 374-382 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kunshige Hamasaki: "The contraction of small arteries in the perimetrium by presurgical medication with the luteirnzing hormone-releasing hormone agonist to patients with leiomyomas : An elctron and immunoelectron microscopy" Fukuoka Acta Medica. 86. 389-397 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshiaki Doi: "The increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta" Virchows Archiv. 428. 367-373 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yosuke Kanazawa: "The roles of the marginal veins in the differentiation of the rat hind limb bud : an immunocytochemical study" Archives of Histology and Cytology. 59. 317-330 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takatoshi Ozaka: "Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body" Anatomical Record. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshiaki Doi: "Histamine release from Weibel-Palade bodies of toad aortas induced by endothelin-1 and sarafotoxin" Anatomical Record. 242. 374-382 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kunshige Hamasaki: "The contraction of small arteries in the perimetrium by presurgical medication with the luteinizing hormonoe-releasing hormone agonist to patients with leiomyomas : An electron and immunoelectron microscopy" Fukuoka Acta Medica. 86. 389-397 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshiaki Doi: "The increase in number of Weibel-Palade bodies and the endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta" Virchows Archives. (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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