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ドーパミン作動性反回入力と興奮性および抑制性入力応答との細胞内クロストーク

Research Project

Project/Area Number 07670047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

NABEKURA Junichi  KYUSHU UNIVERSITY FACULTY OF MEDICINE ASSOCIATE PROF., 医学部, 助教授 (50237583)

Co-Investigator(Kenkyū-buntansha) HOROMOTO Naoki  AKITA UNIVERSITY SCHOOL OF MEDICINE LECTURER, 医学部, 講師 (40243927)
小川 哲朗  秋田大学, 医学部, 教授 (80004555)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsrat / substantia nigra / monoamine / glycine / protein hinase / GTP binding protein / chboride / 神経トレーサー / ドーパミン / 抑制性アミノ酸 / ノルアドレナリン / 蛋白燐酸化酵素
Research Abstract

To investigate the intracellular cross-talk between the receptor for inhibitory amino acid and the monoamine receptor, the following experiments have been performed. Under the anesthesia, rats aged between 12-14 days after birth, were decapitated. The brain slices with 400 micron thickness including substantia nigra were obtained. After the treatment with enzyme, nigra neurons were acutely dissociated. The nystatin perforated patch recording were employed and the modulatory effects of monoamine on the GABA and taurine responses. The application of dopamine suppressed the GABA response. Taurine increased the membrane permeability to chloride ion by the activation of the glycine receptor-channel complex. Noradrenaline potentiated the taurine response, resulting in suppressing the neuronal excitability. The two distinct intracellular pathways were identified in the potentiation of the taurine responses by noradenaline. Activation of alpha2 adrenoceptor which is coupled to pertussis toxin-sensitive GTP binding protein suppresses adenyl cyclase activity, resulting in the decrease of intracellular cyclic AMP and protein kinase A activity. The decrease of protein kinase A activity potentiated the glycine receptor mediated taurine response.
Another pathway in the modulation of taurine response by noradrenaline is as follows. Alpha2 adrenoceptor activated by noradrenaline increased the phospholipase C (PLC) activity through pertussis-toxin sensitive G protein. PLC increased the intracellular protein kinase C activity, resulting in the enhancement of glycine-receptor mediated taurine response. Single channel recording revealed that this enhancement of taurine response by alpha2 adrenoceptor activation was associated with the potentiation of the open probabillity of taurine-activated channel.
These results indicate that noradrenaline enhanced the taurine response by the two distinct intracellular pathways in the rat substantia nigra neurons.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 鍋倉淳一 堀本直幹: "HETEROGENEOUS DEVELOPMENT OF VOLTAGE DEPENDENCY OF NMDA RECEPTUR MEDIATED RESPONSE IN CENTRAL NERVOUS SYSTEM" NEUROBIOLOGY. 3. 293-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鍋倉淳一 上野伸哉 小川哲朗 赤池紀扶: "Colocalization of ATP and nicotinic ACh receptors in the identified vagal preganglinic neurone of rat" Journal of Physiology. 489. 519-527 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 堀本直幹 鍋倉淳一: "DEVELOPMENTAL CHANGES IN ARACHIDONIC ACID POTENTIATION OF NMDA CURRENTS IN CORTICAL NEURONES" NEUROREPORT. 7. 2463-2467 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 浜野浩二、鍋倉淳一 西川正純 小川哲朗: "DOCOSAHEXAENOIC ACID REDUCES GABA RESPONSE IN SUBSTANTIA NIGRA NEURON OF RAT" JOURNAL OF NEUROPHYSIOLOGY. 75. 1264-1270 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鍋倉淳一 大村知広 赤池紀扶: "ALPHA2 ADRENOCEPTOR POTENTIATES GLYCINE RECEPTOR-MEDIATED TAURINE RESPONSE THROUGH PROTEIN KINASE A IN RAT SUBSTANTIA NIGRA NEURONS" JOURNAL OF NEUROPHYSIOLOGY. 76. 2447-2454 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鍋倉淳一 ,大村知広,堀本直幹 小川哲朗 赤池紀扶: "ALPHA1 ADRENOCEPTOR ACTIVATION POTENTIATES TAURINE RESPONSE MEDIATED BY PROTEIN KINASE C IN SUBSTANTIA NIGRA NEURONS" JOURNAL OF NEUROPHYSIOLOGY. 76. 2455-2460 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nabekura, J.Ueno, S., Ogawa, T., andAkaike, N.: "Colocalization of ATP and nicotinic ACh receptors in the identified vagal preganglionic neurone of rat." Journal of Physiology. 489. 519-527 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamano, H., Nabekura, J., Nishikawa, M.and Ogawa, T.: "Docosahexaenoic acid reduces GABA response in substantia nigra neuron of rat." Journal of Neurophysiology. 75. 1264-1270 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nabekura, J., Horimoto, N., Ogawa, T., & Akaike, N.: "Ontogeny of blocking effect of extracellular Mg^<2+> on NMDA response and the influence of input-output organization." International Academy for Biomedical and Drug Research. 11. 27-32 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nabekura, J.and Horimoto, N.: "Heterogeneous development of voltage dependency of NMDA receptor-mediated response in central nervous system." Neurobiology. 3. 293-298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nabekura, J., Omura, T., & Akaike, N.: "Alpha2 adrenoceptor potentiates glycine recepotor-mediated taurine response through protein kinase A in rat substantia nigra neurons." Journal of Neurophysiology. 76. 2447-2454 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nabekura, J., Omura, T., Horimoto, N., Ogawa, T., & N.Akaike: "Alpha1 adrenoceptor activation potentiates taurine responses mediated by protein kinase C in substantia nigra neurons." Journal of Neurophysiology. 76. 2455-2460 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horimoto, N., Nabekura, J., & Ogawa, T.: "Developmental change in arachidonic potentiation of NMDA currents in cortical neurons." Neuroreport. 7. 2463-2467 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horimoto, N., Nabekura, J., & Ogawa, T.: "Arachidonic acid activation of potassiumchannel in rat visual cortex neurons." Neuroscience. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鍋倉淳一 堀本直幹: "HETEROGENEOUS DEVELOPMENT OF VOLTAGE DEPENDENCY OF NMDA RECEPTOR MEDIATED RESPONSE IN CENTRAL NERVOUS SYSTEM" NEUROBIOLOGY. 3. 293-298 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鍋倉淳一 上野伸哉 小川哲朗 赤池紀扶: "Colocalization of ATP and nicotinic ACh receptors in the identified vagal preganglinic neurone of rat" Journal of Physiology. 489. 519-527 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀本直幹 鍋倉淳一: "DEVELOPMENTAL CHANGES IN ARACHIDONIC ACID POTENTIATION OF NMDA CURRENTS IN CORTICAL NEURONES" NEURO REPORT. 7. 2463-2467 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 浜野浩二,鍋倉淳一 西川正純 小川哲朗: "DOCOSAHEXAENOIC ACID REDUCES GABA RESPONSE IN SUBSTANTIA NIGRA NEURON OF RAT" JOURNAL OF NEUROPHYSIOLOGY. 75. 1264-1270 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鍋倉淳一 大村知広 赤池紀扶: "ALPHA2 ADRENOCEPTOR POTENTIATES GLYCINE RECEPTOR-MEDIATED TAURINE RESPONSE THROUGH PROTEIN KINASE A IN RAT SUBSTANTIA NIGRA NEURONS" JOURNAL OF NEUROPHYSIOLOGY. 76. 2447-2454 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鍋倉淳一,大村知広,堀本直幹 小川哲朗 赤池紀扶: "ALPHA1 ADRENOCEPTOR ACTIVATION POTENTIATES TAURINE RESPONSE MEDIATED BY PROTEIN KINASE C IN SUBSTANTIA NIGRA NEURONS" JOURNAL OF NEUROPHYSIOLOGY. 76. 2455-2460 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Junichi Nabekura: "Colocalization of ATP and nicotinic ACh receptors in the identified vagal preganglionic neurone of rat." Journal of Physiology. 489. 519-527 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Junichi Nabekura: "Heterogeneous development of voltage dependency of NMDA receptor-mediated response in central nervous system" Neurobiology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroshi Hamano: "Docosahexaenoic acid reduces GABA response in substantia nigra neuron of rat" Journal of Neurophysiology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鍋倉淳一: "視床下部および辺縁系におけるステロイドホルモンの興奮性調節機構" ホルモンと臨床. 43. 43-44 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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