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ION TRANSPORT,LIPID METABOLISM AND HEAT PRODUCTION DURING COLD ACCLIMATION IN RAT BROWN ADIPOSE TISSUE CELLS.

Research Project

Project/Area Number 07670095
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionHYOGO COLLEGE OF MEDICINE

Principal Investigator

HORI Seiki  HYOGO COLL.MED., PROFESSOR, 医学部, 教授 (80068452)

Co-Investigator(Kenkyū-buntansha) HORI Kazuko  HYOGO COLL.MED., ASSISTANT PROFESSOR, 医学部, 講師 (30068514)
TSUJITA Jyunzo  HYOGO COLL.MED., RESEARCHER, 医学部, 助手 (30104235)
SASAKI Sadao  HYOGO COLL.MED., PROFESSOR, 医学部, 助教授 (20104276)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBROWN ADIPOCYTE / HEAT PRODUCTION / INTRACELLULAR ION CONTENT / COLD ACCLIMATION / THERMOGENIN / LIPID METABOLISM
Research Abstract

The effects of warm or cold (8w) acclimation and reacclimation (cold, 4w : warm, 4w) on the intracellular ion transport, lipid metabolism and heat production of the brown adipose tissue cells were studied. The cells were investigated by electron probe X-ray microanalysis and organelles by the patch clamp technique.
The high voltage electron micrographs of the cells of cold acclimated rats revealed mitochondrial swelling and a decrease and/or deformation of lipid droplets of tissue cells. X-ray microanalysis of the mitochondria of the cold acclimated cells showed lower K and Cl, and higher Ca concentrations than those of the warm acclimated ones. The Ca of the reacclimated rat decreased to the level of the warm acclimated rat. The reconstituted membrane of the organelle was investigated by patch clamp to measure the voltage and-or H+ sensitive currents during cold acclimation. The experiments revealed the presence of a small and a relatively large conductance that correspond to the Cl-channel and the Ca2+dependent K+channel in the inner membrane of the organelle of the cells during cold acclimation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

Research Products

(24 results)

All Other

All Publications (24 results)

  • [Publications] Hori,S: "Adaptation to heat." Jpn. J. Physiol.45(6). 921-946 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki,S., Nakagaki,I., Kondo,K.,Hori,S.: "Changes in element concentrations induced by agoinst in pig panctratic acinar cells." Pflugers Arch.,. 432. 538-545 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki,S.,Nakagaki,I.,Hori,K.,Tsujita,J.,Hori,S .,Yahata,T.,Kuroshima,A: "Intracellular ion transport in mitochondrial membrance during cold acclimation in rat brown adipose tissue." Ed. Nagasaka,T. & Milton,A.S.In : Body temperature and melabolism,. IPEC Inc.,Tokyo,. 203-206 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki,S.,Nakagaki,I.,Kaya,M.,Takemura,M.,Koyama,K.,Tsujita,J.Hori,S.,Yahata,T.,Kuroshima,A.: "Ion transport,lipid metabolism and heat production in mitochondrial membrane during cold acclimation in rat brown adipose tissue cells." Jpn. J. Physiol..46Suppl,.S214 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中垣育子、佐々木貞雄、高屋豊、賀屋光晴、武村正徳、他: "ラット褐色脂肪細胞の細胞内イオン変動に及ぼす寒冷曝露の影響" 日本生気象学会雑誌、. 32(3). S46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 堀和子、佐々木貞雄、中垣育子、辻田純三、小山勝弘、他: "寒冷順化にともなうラット褐色脂肪細胞の形態およびサーモジェニン、細胞内イオンの動態" 第18回日本分子生物学会. 451 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hori, S.: "Adaptation to heat." Jpn.J.Physiol.45(6). 921-946 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, S., Nakagaki, I., Kondo, H., Hori, S.: "Changes in element concentrations induced by agonist in pig panctratic acinar cells." Pflugers Arch.432. 538-545 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, S,Nakagaki, I., Kaya, M., Takemura, M., Koyama, K., Tsujita, J., Hori, S., Yahata, T., Kuroshima, A.: "Ion transport, lipid metabolism and heat production in mitochondrial membrane during cold acclimation in rat brown adipose tissue cells." Jpn.J.Physiol.46Suppl.S214 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, S., Nakagaki, I., Hori, K., Tsujita, J., Hori, S., Yahata, T., Kuroshima, A.: "Intracellular ion transport in mitochondrial membrane during cold acclimation in rat brown adipose tissue." Ed.Nagasaka, T.& Milton, A.S.In : Body temperature and metabolism.IPEC Inc., Tokyo. 203-206 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hori, S., Tanaka, N.: "Effect of physical training and heat acclimation on the physiological responses of men during exercise in heat." Ed.Nagasaka, T.& Milton, A.S.In : Body temperature and metabolism. IPEC Inc., Tokyo. 113-116 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsujita, J., Umebayashi, Y., Hori, S.: "The effects of ambient temperature and wind on the physiological responses of men during treadmill running." Ed.Nagasaka, T.& Milton, A.S.In : Body temperature and metabolism. IPEC Inc., Tokyo. 117-120 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hori,S.: "Adaptation to heat." Jpn.J.Physiol.45(6). 921-946 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,S,Nakagaki.I.,Kaya,M.,Takemura,M.,Koyama,K.,Tsujita,J.Hori,S.,Yahata,T.,Kuroshima,A.: "Ion transport,lipid metabolism and heat production in mitochondrial membrane during cold acclimation in rat brown adipose tissue cells." Jpn.J.Physiol.,. 46Suppl.,. S214- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,S.,Nakagaki,I.,Kondo,K.,Hori,S.: "Changes in element concentrations induced by agonist in pig panctratic acinar cells." Pflugers Arch.,. 432. 538-545 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,S.,Nakagaki,I.,Hori,K.,Tsujita,J.,Hori,S.,Yahata,T.,Kuroshima,A.: "Intracellular ion transport in mitochondrial membrane during cold acclimation in rat brown adipose tissue." Ed.Nagasaka,T.& Milton,A.S.In : Body temperature and metabolism,. IPEC Inc.,Tokyo,. 203-206 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中垣育子、佐々木貞雄、高屋豊、賀屋光晴、武村正徳、他: "ラット褐色脂肪細胞の細胞内イオン変動に及ぼす 寒冷曝露の影響" 日本生気象学会雑誌、. 32(3). S46- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀和子、佐々木貞雄、中垣育子、辻田純三、小山勝弘、他: "寒冷順化にともなうラット褐色脂肪細胞の形態およびサーモジェニン、細胞内イオンの動態" 第18回日本分子生物学会、. 451- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seiki Hori: "Adaptation to Heat" Jpn. J. Physiol.45(6). 921-946 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasaki, S. Nakagaki, I. Hori, K., Tsujita, J., Hori,S. et al.: "Ion transport in mitochondrial memlirane during coed acclimation in rat luroun adipose tissue." The proceedings of the second joint meetings of the physislogical eaietis of Japan and U. K and Eire.12 : Body temperatren and metabolism.(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hori S, Tanaka N.: "Effect of physical training and heat acclimation on physiological responses of men during exerise in heat." The proceeings of the second joint meeting of the physioligial societis of Japan, UK and Eire 12 : Body temperature and metabolism.(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀和子,佐々木貞雄,中垣育子,辻田純三,堀清記 et al.: "寒冷馴化にともなうラット褐色脂肪細胞の形態およびサーモゲニン、細胞内イオンの動態" 第18回日本分子生物学会大会 講演要旨集. 451- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中垣育子,佐々木貞雄,高尾豊,賀尾光晴,武村政徳,堀清記 他.: "ラット褐色脂肪細胞の細胞内イオン変動に及ぼす寒冷曝露の影響" 日本生気象学会雑誌. 32(3). 46- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsujita J., Umebayashi K, Hori S.: "Effects of ambient tenaperature and wind on physiological responses of men during treadmill running." The proceesting of the second joint meeting of the physiolojical societies of Japan UK and Tire. Body temperature and metalabism.(in press). (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-03-31   Modified: 2016-04-21  

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