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Regulation of glycolysis by a novel brain-type isozyme of fructose 6-phosphate 2kinase/fructose 2,6-bisphosphatase

Research Project

Project/Area Number 07670157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOSAKA MEDICAL SCHOOL

Principal Investigator

WATANABE Fusao  OSAKA MEDICAL SCHOOL,DEPARTMENT OF CHEMISTRY ; ASSOCIATE PRPFESSOR, 教養部, 講師 (40183719)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsglycolysis / fructose 2,6 bisphospate / brain / fructose 6-phosphate 2-kinase / fructose 2,6-bisphosphatase / alternative splicing / ホスホフルクトキナーゼ / フルクトース2.6二燐酸合成酵素 / 分子生物学
Research Abstract

Fructose 6-phospate, 2-kinase/fructose 2,6-bisphosphatase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, which is the most potent activator of glycolysis. We have shown previouslt the occurrence of a partial cDNA (RB7) encoding the catalytic core domain of a novel brain type isozyme. To elucidate the full-length sequence of RB7 cDNA,we have carried out cDNA cloning using 3'- and 5'- rapid amplification cDNA ends method and have isolated eight isoforms from rat brain. The cDNA sequences encoding the 5'-untranslated region, the amino-terminal domain, and the catalytic core domain were identical among all th isoforms. However, heterogeneity of the carboxyl-terminus was found by sequence analysis. This heterogeneity was shown not to have resulted from transcription of multiple genes, as Southern blot and genomic sequence analysis revealed that the gene was a single copy in the rat genome. It is likely that these transcripts represent splice variants of the gene. High-level expression of the gene was also observed northern blot analysis in skeletal muscle. However, the pattern of alternative splicing was different from that of brain, and only four isoforms were detected in skeletal muscle.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] WATANABE, F. et al: "Enzymatic and fluorescence studies of four single-tryptophan mutants of rat testis fructose 6-phosphate, 2-kinase:fructose 2,6-bishosphatase" Protein Sci.5. 904-913 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] WATANABE, F, et al: "Novel Isoforms of rat brain fructose 6-phosphate, 2-kinasel frutose 2,6-bisphosphatase are generated by tissue-specific alterative splicing" J. Neurochem.69(In press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe, F.et al.: "Enzymatic and fluorescence studies of four single-tryptophan mutants of rat testis fructose 6-phosphate, 2-kinase : fructose 2,6-bisphosphatase." Protein Sci.5. 904-913 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe, F.et al.: "Novel isoforms of rat brain fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase are generated by tissue-specific alternative splicing" J.Neurochem.69 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] WATANABE,F.et al: "Navel Isoforms of Ret Brain Fructose phosphate,2-Kinase 1 Fructose 2,6-bisphosphatase Are Generated by Tissue-Specific Alteratiue Splicing" Journal of Neurochemistry. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 渡邊 房男: "フルクトース2、6二燐酸合成酵素の新しい脳型アイソザイム" 生化学. 67. 864 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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