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DETERMINATION OF STRUCTURE OF AMYLOIDOGENIC IMMUNOGLOBULIN LIGHT CHAIN AND APPLICATION TO MOLECULAR DIAGNOSIS

Research Project

Project/Area Number 07670200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionHAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

MIURA Katsutoshi  HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF PATHOLOGY,UNIVERSITY HOSPITAL,ASSISTANT PROFESSOR, 医学部附属病院, 助教授 (20173974)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsAMYLOID / IMMUNOGLOBULIN / POLYNEUROPATHY / INFLAMMATION / HUMAN PAPILLOMA VIRUS / PCR / IN SITU HYBRIDIZATION / 扁桃 / アミロイドーシス / エドマン分解 / PCR反応
Research Abstract

Amyloid fibrils were extracted in water and dissolved in 7M urea. Main paptide was separated with SDS polyacrylamide gel electrophoresis and transfered on PVDF membrane. Main peptides were cut from the membrane and subjected to N-terminal amino acid sequence analysis. A pulmonary nodular amyloidosis with systemic involvement was determined as lambda III type AL-amyloidosis, while a tonsillar amyloidosis of localized type was lambda VI type AL-amyloidosis.
A case of familial amyloidosis with polyneuropathy had amyloid fibrils composed of transthyretin variant with substitution leucine for valine at position 30.
Acute phase proteins such as SAA are produced in liver induced by cytokines from inflammatory sites and enter into the blood circulation. They reach the inflammatory sites, act against tissue damage, and suppress the inflammation. I reviewed this system and summarized in a chapter of a book.
To investigate a association of HPV with tumorgenesis in unterine cervix and head and neck region, we extracted DNA from tumors, amplyfied HPV genome with PCR method, and detected the localization of HPV genome in the tumor by in situ hybridazation (ISH). In malignant lesions of uterine cervix, HPV genomes of malignant type were detected in about 90% most of which were not detected in ISH.In head and neck, esophageal squamous cell carcinoma (SCC) and tonsillar SCC contained HPV52b and HPV 16 respectively.
We reported cases of papillary adenocarcinoma of mid-ear, metastatic sebacious carcinoma to the parotid gland, hyalinizing clear cell carcinoma of minor salivcary gland which recurred several times in 13 years, subglottal squamous cell carcinoma with hematogenous metastatis to thyroid, and verrucous carcinoma of temporal bone.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 清水秀昭: "異型トランスサイレチン(Val^<30>→Leu)沈着による家族性アミロイドボリニュロパチーの剖検例" BRAIN and NERVE (脳神経). 48・2. 175-178 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 三浦克敏: "内リンパ嚢原発が疑われるlow-grade papillary adenocarcinomaの1例-文献的考察" 病理と臨床. 14・2. 251-258 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 三浦克敏: "頭頸部病変におけるパラフィン切片からのHPVゲノムの検出と型の決定" 日本病理学会会誌. 85・1. 170- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 原田英一: "子宮頸部病変におけるIn situ hydridizationを用いたHPVの検出" 日本臨床細胞学会雑誌. 34・2. 243- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 三浦克敏: "13年間再発を繰り返す小唾液腺由来のHyalinizing clear cell carcinoma" 病院病理. 13・2. 140- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 三浦克敏: "耳下腺浅葉に転移したマイボ-ム腺由来の皮脂腺癌の1例" 病院病理. 14・1. 54- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 三浦克敏: "現代病理学大系5、炎症と感染、急性期蛋白" 中山書店, 428 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimizu H,Ishikawa K,Kobayashi H,Murakami T,Nakasato M,Miura K,Atsumi T :"An autopsy case of familial amyloidotic polyneuropathy due to deposition of variant transthyretin (va130*Leu)." Noshinnkei. 48(2). 175-178 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miura K,Tsutsui Y :"A case report of low-grade papillary adenocarcinoma of probable endolymphatic sac origin with a review of the literature and differential diagnosis." Byorito rinsho. 14(2). 251-258 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miura K :"Hyalinizing clear cell carcinoma of minor salivary gland showing repeated recurrence in 13 years." Byoinn byori. 13(2). 140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miura K :"A case of sebacious carcinoma of Meibomian gland metastatic to superficial parotid gland." Byoinn byori. 14(1). 54 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miura K :"Invasive temporal bone tumor of papillo-tubular structures without atypia." Byoinn byori. 13(1). 65 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miura K :"Detection and typing of HPV genome from paraffin sections of head and neck lesion." Nippon byouri gakkai kaishi. 85(1). 170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Harada H,Kawabata Y,Nakajima A,Miura K :"Detection of HPV genome in uterine cervical lesions using in situ hybridization." Nippon rinnshou saibou gakkai kaishi. 34(2). 243 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saegusa T,Ito K,Oba N,Matsuda M,Kojima K,Tohyama K,Matsumoto M,Miura K,Suzuki H.: "Enlargement of multiple cavernous hemangioma of the liver in association with pregnancy." Internal Medicine. 34. 207-211 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masui T,Takahashi M,Miura K,Naito M,Tawarahara K :"Cardiac myxoma, identification of intratumoral hemorrhage and calcification on MR images." AJR.164. 850-852 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hoshino T,Amano H,Morita K,Miura K.: "Two cases of verrucous carcinoma" Ji-koutou-kei. 67(9). 864-869 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mukoudaka H,Miura K et al :"Subglottal carcinoma with hematogenous metastasis to thyroid." Johns. 11(5). 769-774 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MIURA K.: Inflammation and infection. Acute phase protein.Current encyclopedia of pathology 5, Nakayama-shotten, 207-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 清水秀昭: "異型トランスサイレチン(Val^<30>→Leu)沈着による家族性アミロイドボリニュロパチーの剖検例" BRAIN and NERVE(脳神経). 48・2. 175-178 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "内リンパ嚢原発が疑われるlow-grade papillary adenocarcnomaの1例-文献的考察" 病理と臨床. 14・2. 251-258 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "頭頚部病変におけるパラフィン切片からのHPVゲノムの検出と型の決定" 日本病理学会会誌. 85・1. 170- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 原田英一: "子宮頚部病変におけるIn situ hybridizationを用いたHPVの検出" 日本臨床細胞学会雑誌. 34・2. 243- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "13年間再発を繰り返す小唾液腺由来のHyalinizing clear cell carcinoma" 病院病理. 13・2. 140- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "耳下腺浅葉に転移したアイボ-ム腺由来の皮脂腺癌の1例" 病院病理. 14・1. 54- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "現代病理学大系5,炎症と感染,急性期蛋白" 中山書店, 428 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦克敏: "炎症と感染" 中山書店, 428 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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