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THE MOLECULAR MECHANISM OF ASSOCIATION OF EB VIRUS WITH LUNG

Research Project

Project/Area Number 07670207
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionSAPPORO MEDICAL UNIVERSITY

Principal Investigator

KASAI Kiyoshi  SAPPORO MEDICAL UNIVERSITY,SCHOOL OF HEALTHSCIENCES,ASSOCIATE PROFESSOR, 保健医療学部, 助教授 (70175272)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsLung cancers / Epstein-Barr virus / in situ hybridization / oncogenesis by virus / in situ hybridization / ウィルス発癌
Research Abstract

To evaluate the presence of Epstein-Barr virus (EBV) in the lung cancers, and tissues, 188 lung cancers, 67 normal lung, 50 pneumonias and 16 atypical epithelial lung tissues were examined using in situ hybridization (ISH) method and polymerase chain reaction (PCR). Although EBV-RNA was not detected in all the normal lung and the precancerous lesion and most lung cancers, it was found to be positive for the bronchiolar epithelium of 8 interstitial pneumonias, indicating the bronchiolar epithelium to be a target of EBV and local immunological disturbance to possibly relate to EBV infection in interstitial pneumonias. EBV-RNA and EBV-DNA were shown to be positive for 2 lymphoepitheliallike carcinoma of the lung (LELC) and 4 non-small cell lung cancers. Three EBV-associated lung cancers were evaluated for point mutation in codon 12 and codon 61 of the K-ras and Ha-ras oncogenes and mutations in the p53 tumor suppressor gene by PCR and SSCP-PCR followed by direct sequencing. These mutations in the K-ras and Ha-ras genes and p53 genes were not observed in the 3 EBV-associated lung cancers.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 笠井潔、菊地浩吉: "免疫関連遺伝子の可視化とその応用-in situ hybridizationを中心として-" 免疫Immunology Frontier. 6. 61-70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 松浦晃洋、笠井潔、杵渕幸、菊地浩吉: "CDI抗原の構造、発現、機能-新しい抗原提示分子としての役割-" Annual Review 免疫 1997. 183-191 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinichiro Kon,Kiyoshi Kasai: "Primary gastric T cell lymphoma with Epstein-Barr virus infection." Tumor Res. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 笠井潔: "生体反応の分子情報学" 中外医学社(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kasai K,Sato Y,Kameya T,Inoue H,Yoshimura H,Kon S,Kikuchi K.: "Incidence of latent infection of Epstein-Barr virus in lung cancers-An analysis of EBERI expression in lung cancers by in situ hybridization." J Pathol. 174. 257-265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torigoe, T., Sato, M., Kasai, K., Kon, S., Sano, S., Furuya, T., Ishii, Y.: "Detection of Epstein-Barr virus in gastric adenocarcinoma by PCR and in situ hybridization and immunohistochemical analysis of tumor infiltrating lymphocytes." Ann Res Rep. SDF Sapporo Hosp. 35. 28-35 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kiyoshi Kasai, Akihiro Matsura, Kokichi Kikuchi, Yuuji Hashimoto, Shingo Ichimiya: "Localization of rat CD1 trans-cripts and protein in rat tissues-An analysis of rat CD1 expression by in situ hybridization and immunohistochemistry." Clin Exp Immunol. 108 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinichiro Kon, Kiyoshi Kasai, Jun Yamaguchi: "Primary gastric T cell lymphoma with Epstein-Barr virus infection." Tumor Res. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 笠井潔、菊地浩吉: "免疫関連遺伝子の可視化とその応用-in situ hybridizationを中心として-" 免疫Immunology Frontier. 6. 61-70 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松浦晃洋、笠井潔、杵渕幸、菊地浩吉: "CD1抗原の構造、発現、機能-新しい抗原提示分子としての役割-" Annual Review 免疫 1997. 183-191 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinichiro Kon,Kiyoshi Kasai: "Primary gastric T cell lymphoma with Epstein-Barr virus infection." Tumor Res.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 笠井潔: "生体反応の分子情報学" 中外医学社(印刷中), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 笠井潔、菊地浩吉: "免疫関連遺伝子の可視化とその応用-in situ hybridizationを中心として-" 免疫Immunology Frontier. 6. 61-70 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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