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A ROLE OF AMYLOID P COMPONENT AND NUCLEOSOME IN THE INITIATION OF LUPUS NEPHRITIS

Research Project

Project/Area Number 07670236
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNiigata University School of Medicine

Principal Investigator

MORIOKA Tetsuo  NIIGATA UNIVERSITY SCHOOL OF MEDICINE Dept.Cellular Physiology Associate Professor, 医学部, 助教授 (00210146)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Fujio  NIIGATA UNIVERSITY SCHOOL OF MEDICINE Dept.Cell Biology Professor, 医学部, 教授 (40012728)
OITE Takashi  NIIGATA UNIVERSITY SCHOOL OF MEDICINE Dept.Cellular Physiology Professor, 医学部, 教授 (60018744)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordslupus nephritis / nucleosome / amyloid P component / histones / DNA / immune complexes / SLE / glomerular basement membrane (GBM) / 血清アミロイドP蛋白
Research Abstract

With aid of the above grant, we have researched and reported on the mechanism of initiation of lupus nephritis. We have obtained the following results during the period of research project :
(1) Antibodies from serum of SLE patients can form soluble histone-DNA-anti-DNA immune complexes that bind to the glomerular capillary wall in vivo.
(2) Amyloid P component is a constituent of normal human glomerular basement membrane
(3) DNA and nucleosome core particles could bind to human GBM in the presence of Ca^<++.>
(4) Treatment of GBM with trypsin resulted the loss of amyloid P component on the GBM.The binding of DNA and nucleosome core particles to the GBM were reduced when GBM were treated with trypsin.
These results suggested that DNA and nucleosome core particles could bind to human GBM and this may cause the initiation of lupus nephritis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] MORIOKA, TETSUO: "Anti-DNA antibody derived from an SLE patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo" Clinical and Experimental Immunology. 104. 92-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] FUJIGAKI, YOSHIHIDE: "Structural contimuity of filtration slit (slit diaphragm) to plasma membrane of podocyte" Kidney International. 50. 54-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] NARISAWA-SAITO, MAKO: "Thy-1 molecule associate with protein tyrosine kinase in rat mesangial cells" Clinical and Experimental Immunology. 106. 86-90 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] OITE, TAKASHI: "A specific Thy-1 molecular epitope expressed on rat mesangial cells" Experimental Nephrology. 4. 350-360 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] WOITAS, REINER: "Influence of isoelectric point on glomerular deposition of antibodies and immune complexes" Nephron. 74. 713-719 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森岡、哲夫: "ループス腎炎発症に果たすアポトーシスの役割" 医学の歩み. 178. 781-785 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 追手、巍: "糸球体における内皮細胞とメサンギウム細胞の接着様式" 生理学研究所年報. 17. 267-268 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Q.Cheng, et al.: "Progressive renal lesions induced by administration of monoclonal antibody 1-22-3 to unilaterally nephrectomized rats" Clinical and Experimental Immunology. 102. 181-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Oite, et.al.: "Efficient isolation of rat endothelial cells by elimination of contaminating cells with a monoclonal antibody" Microvascular Research. 50. 113-118 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Oite, et.al.: "Modulation of mesangial cell behavior by communication circuits between extrinsic and intrinsic cells or cells and matrices" Niigata Symposium of Nephrology 1995. Recent Advances in Molecular Nephrology.edited by M.Arakawa and Y.Nakagawa.Kohko-Do, Niigata. 98-112 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R.Woitas, et al.: "Influence of isoelectric point on glomerular deposition of antibodies and immune complexes" Nephron. 74. 713-719 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Morioka, et.al.: "Anti-DNA antibody derived from an SLE patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo" Clinical and Experimental Immunology. 104. 92-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Oite, et al.: "A specific Thy-1 molecular epitope expressed on rat mesangial cells" Experimental Nephrology. 4. 350-360 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fujigaki, et al.: "Structural continuity of filtration slit (slit diaphragm) to plasma membrane of podocyte" Kidney International. 50. 54-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Narisawa-Saito, et al.: "Thy-1 molecule associate with protein tyrosine kinase in rat mesangial cells" Clinical and Experimental Immunology. 106. 86-90 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] CHENG,QIAN: "Progressive renal lesions induced by administration of monoclonal antibody 1-22-3 to unitaterally nephrectomized rats" Clincal and Experimental Immunology. 102. 181-185 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] OITE,TAKASHI: "Efficient isolation of rat endothelial cells by elimination of contaminating cells with a monoclonal antibody" Microvascular Research. 50. 113-118 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 追手、巍: "糸球体における内皮細胞とメサンギウム細胞の接着様式" 生理学研究所年報. 16. 297-298 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森岡、哲夫: "ループス腎炎発症に果たすアポトーシスの役割" 医学の歩み. 178. 781-785 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] NARISAWA-SAITO,MAKO: "Thy-1 molecule associate with protein tyrosine kinase in rat mesangial cells" Clinical and Experimental Immunology. 106. 86-90 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] OITE,TAKASHI: "A specific Thy-1 molecular epitope expressed on rat mesangial cells" Experimental Nephrology. 4. 350-360 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] MORIOKA,TETSUO: "Anti-DNA antibody derived from an SLE patient froms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo" Clinical and Experimental Immunology. 104. 92-96 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] FUJIGAKI,YOSHIHIDE: "Structural contimuity of filtration slit (slit diaphragm) to plasma membrane of podocyte" Kidney International. 50. 54-62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 追手、巍: "糸球体における内皮細胞とメサンギウム細胞の接着様式" 生理学研究所年報. 17. 267-268 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] OITE,TAKASHI: "Recent Advances in Molecular Nephrology" M.ARAKAWA & NAKAGAWA Eds., 119 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Cheng, Qian: "Progressive renal lesions induced by administration of monoclonal antibody 1-22-3 to unitaterally nephrectomized rats." Clinical and Experimental Immunology. 102. 181-185 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oite, Takashi: "Efficient isolation of rat endothelial cells by elimination of contaminating cells with a monoclonal antibody." Microvascular Research. 50. 113-118 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Morioka, Tetsuo: "Anti-DNA antibody derived from an SLE patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo" Clinical and Experimental Immunology. 104. 92-96 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujigaki, Yoshihide: "Structural contimuity of filtration slit(slit diaphragm)to plasma membrane of podocytes" Kidney International. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oite, Takashi: "A specific Thy-1 molecular epitope expressed on rat mesangial cells." Experimental Nephrology. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oite, Takashi: "Recent Advances in Molecular Nephrology" M. Arakawa & Y. Nakagawa Eds., 119 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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