• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional significance of erythroblastic island composed of a central macrophage and surrounding erythroblasts.

Research Project

Project/Area Number 07670262
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKAWASAKI MEDICAL SCHOOL

Principal Investigator

SADAHIRA Yoshito  Kawasaki Med.Sch., Pathology, assistant professor, 医学部, 講師 (30178694)

Co-Investigator(Kenkyū-buntansha) YASUDA Tatsuji  Okayama Univ.Sch.of Med., Professor, 医学部, 教授 (30092357)
WADA Hideho  Kawasaki Med.Sch., Hematology, assistant professor, 医学部, 講師 (70191830)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMacrophage / Erythropoiesis / Liposome / Erythropoetin / Spleen / Liver / 肝臓 / マウス / 造血環境 / エリスロポエチン / C-kit ligand / 造血微小環境
Research Abstract

In the hematopoietic tissues, erythroid cells closely associate with resident macrophages, forming erythroblastic islands via adhesion molecules. To elucidate the role of macrophages in erythropoiesis, we selectively abrogated resident macrophages of splenic red pulp and liver of phlebotomized mice, by injection with dichrolomethylene-bisphosphonate encapsulated in multilamellar liposomes (C12MDP-liposomes). In the spleen, no erythropoietic activity occurred until 5 days after the treatment. The colony assay revealed that the erythroid suppression occurred at the level of CFU-E.The splenic erythropoietic activity gradually developed from day 6 after the treatment, when F4/80^+ macrophages began to appear in the red pulp. In contrast to the splenic response, extramedullary erythropoiesis occurred in the liver even while F4/80^+ macrophages (Kupffer cells) were eliminated by injection of C12MDP-liposomes. RT-PCR study demonstrated no expression of erythropoietin (EPO) in the liver, spleen, and bone marrow both of the control phlebotomized mice and the phlebotomized mice injected with C12MDP-liposomes. EPO expression of the kidney was enhanced by phlebotomy, but that was suppressed by the injection of C12MDP-liposomes. Injection of recombinant human EPO (rh-EPO) restored splenic erythropoiesis partially and enhanced hepatic erythropoiesis. Thus, poor erythropoietic response in spleen of the phlebotomized mice injected with C12MDP-liposomes might be induced by low erythropoietin production of the kidney and abrogation of macrophages.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 定平吉都: "造血組織定住性マクロファージの形態と機能" 日本網内系学会会誌. 35・5. 261-270 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 定平吉都: "造血におけるマクロファージ" 病理と臨床. 16・9(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshito Sadahira: "Morphology and function of resicent macrophages in hematopoietic tissues" The Journal of the Japanese Society of Reticuloendothelial System. 35. 261-270 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshito Sadahira: "Macrophage in hematopoiesis" Byohri to Rinshyoh. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 定平吉都: "造血におけるマクロファージ" 病理と臨床. 16・9(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 定平吉都: "造血組織定住性マクロファージの形態と機能" 日本網内系学会会誌. 35. 261-270 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi