| Project/Area Number |
07670267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | NATIONAL INSTITUTE OF NEUROSCIENCE,NCNP |
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Principal Investigator |
HAGIWARA Yasuko NATIONAL INSTITUTE OF SECTION CHIEF NUROSCIENCE,NCNP, 精神研究所・モデル動物開発部, 室長 (00175530)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Satoru NATIONAL INSTITUTE OF POST DOCTER FELLOW NUROSCIENCE,NCNP, 神経センター・神経研究所, 研究員
MIZUNO Yuji NATIONAL INSTITUTE OF POST DOCTOR FELLOW NUROSCIENCE,NCNP, 神経センター・神経研究所, 研究員
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Muscular dystrophy / Skeletal muscle / Myoblast transplantation / Dystrophin / Dystrophin-associated proteins / mdx mouse / 組み換えアデノウイルス / mdxマウス / 筋ジストロフィーマウス / ジストロフィン / カベオリン-3 / サルコグリカン |
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| Research Abstract |
Dystropin is a responsible protein of Duchenne muscular dystrophy (DMD) and is present at the protoplasmic surface of normal sarcolemma. Its absencd is observed in DMD and mdx (mouse equivalent to DMD) muscles and is considered to be the cause of muscle fiber degeneration. To construct the subsarcolemmal dystrophin-undercoat in mdx muscle, myoblasts with normal dystrophy gene are injected into mdx mouse muscle. We injected C2 myblasts, a normal mouse muscle cell line, into the muscles of mdx nude mouse. A large number of dystrophin-positive fibers appeared after C2 myoblast transplantation. We examined dystrophin-positive fibers with antidystrophin associated proteins antibodies. We reported that dystrophin-associated proteins are biochemically classified into 3 groups, namely, dystroglycan complex and syntrophin complex. We also cloned a new sarcoglycan (gamma-sarcoglydan) cDNA and showed that the human gamma-sarcoglycan gene was mappetd to chromosome 13q12 and delations that alter its reading frame were identified in three families and one of four sporadic cases of SCARMD.We showed the efficient transduction and expression of lacZ gene in the skeletal muscle of adult C57BL/10ScSn mice after adenovirus-mediated gene transfer. Sixty-two percent of the myofibers in the tibialis anterior (TA) muscle were b-galactosidase-positive after injection of the lacZ gene under the control of the chicken b-actin promoter and the cytomegalovirus enhancer. The transduced gene was preferentially expressed in type IIA and IIX fibers which were richer in oxidative enzymes than type IIB fibers.
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