• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of hematogenous metastasis of human gastric cancer using LacZ gene-tagged tumor cells.

Research Project

Project/Area Number 07670268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

NAKANISHI Hayao  Aichi Cancer Center Research Institute, Laboratory, of Pathology, Section head, 病理学第一部, 室長 (20207830)

Co-Investigator(Kenkyū-buntansha) OGURI Kayoko  National Nagoya Hospital, Clinical Research Institute, Senior Researcher, 臨床研究部, 研究員 (10158826)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGastric Cancer / Human / LacZ gene / Metastasis / Circulating tumor cells / Lewis carcinoma / Syndecan / PCR
Research Abstract

1. We previously developed a transplantable human gastric carcinoma line with high spontaneous lung metastatic potential in nude mice (GLM-1). We further established a new metastatic, human gastric carcinoma line (GLM-2) and a rat prostatic carcinoma cell line transfected with LacZ gene (PLS30LZ). These cell lines circulate in the bloodstream in cell clusters and form micrometastatic foci in the lung after intravascular growth like GLM-1 cells, as demonstrated by X-Gal and BrdU double staining. This is sharp contrast to the present leucocyte migration model in which tumor cells reaching metastatic site first transmigrate through the endothelium and grow in the extravascular space to form metastatic foci. These results suggest that the metastatic process is diverse and there is a molecular mechanism distinct from the leucocyte model in which sLex, sLea-E selectin and integrin-ECM interactions play a major role in the development of metastasis.
2. Using LacZ gene transfected cells, we fou … More nd that mouse Lewis lung carcinoma cells metastasize to the lung in a similar manner to the leucocyte model. Experimental lung metastasis produced by i.v.injection of the tumor cells, however, was inhibited by heparin but not by RGDS peptide derived from fibronectin. Immunohistochemistry using antisyndecan-2 antibody showed the cell surface expression of this proteoglycan in the tumor cells. These results suggest that a non-integrin cell surface receptor for ECM is involved in the arrest and subsequent micrometastasis formation in this model.
3. Establishment of cultured cell line from GLM-1 transplantable line in a nude mouse not feasifle to data. Primary cultured cells cease growth after 5-10 generations in culture. Expression of LacZ gene in the above transfected non-metastatic human gastric carcinoma cell line (HSC-39) is relatively weak and unstable. Immortalization of GLM-1 tumor with SV40 T antigen and LacZ gene transfection using electroporation technique are now on going. Further in vivo studies are needed to clarify the molecular basis of the natural history of human gastric cancer metastasis. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 中西 速夫: "LacZ遺伝子導入による肺微小転移形成過程の解析" The Lung Perspectives. 3(4). 51-54 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Itano, N. et. al.: "Phosphorylation of membrane-intercalated proteoglycan, syndecan-2, expressed in a stroma-inducing clone from a mouse Lewis lung carcinoma." Biochem.J.315. 925-930 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H. et. al.: "Establishment and characterization of three androgen-independent, metastatic carcinoma cell lines from 3,2-dimethyl-4-aminobiphenul-induced prostatic tumors in F344rats." Jpn.J. Cancer Res.87(12). 1218-1226 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, K. et. al.: "Growth characteristics in the initial stage of micrometastasis formation by bacterial lacZ gene-tagged rat prostatic adenocarcinoma cells." Jpn.J. Cancer Res.87(12). 1199-1203 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, K. et. al.: "Sequential observation of micrometastasis formation by bacterial lacZ gene-tagged Lewis Lung carcinoma cells." Cancer Letters. 112. 191-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H. et.al.: "Detection of carcinoembryonic antigen-expressing free tumor cells in peritoneal washes from patients with gastric carcinoma by the polymerase-chain reaction." Jpn.J.Cancer Res.88. 687-692 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H. et.al.: "Detecion of tumor cells in blood and peritoneal lavage fluid of colorectal and gastric cancer patients by nested primer RT-PCR analysis. in ″Recent advances in gastroenterological carcinogenesis″ ed. E.Tahara." Monduzzi Editore. Bologna. (Italy)., 853-856 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Itano, N.et.al.: "Phosphorylation of a membrane-intercalated proteoglycan, syndecan-2, expressed in a stroma-inducing clone from a mouse Lewis lung carcinoma." Biochem.J.315. 925-930 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H.et.al.: "Establishment and characterization of three androgen-independent, metastatic carcinoma cell lines from 3,2'-dimethyl-4-aminobiphenyl-induced prostatic tumors in F344 rats." Jpn.J.Cancer Res.87. 1218-1226 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, K.et.al.: "Growth characteristics in the initial stage of micrometastasis formation by bacterial LacZ gene-tagged rat prostatic adenocarcinoma cells." Jpn.J.Cancer Res.87. 1227-1234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H.et.al.: "Recent advances in gastroenterological carcinogenesis.I" Monduzzi Editore. 853-856 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, K.et.al.: "Sequential observation of micrometastasis formation by bacterial LacZ gene-tagged Lewis lung carcinoma cells." Cancer Letters. 112. 191-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H.et.al.: "Detection of carcinoembryonic antigen-expressing free tumor cells in peritoneal washes from patients with gastric carcinoma by the polymerase-chain reaction." Jpn.J.Cancer Res.88. 687-692 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Itano,N.et.al.: "Phosphorylation of membrane-intercalated proteoglycan,syndecan-2,expressed in a stroma-inducing clone from a mouse Lewis lung carcinoma." Biochem.J.315. 925-930 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakanishi,H.et.al.: "Establishment and characterization of three androgen-independent,metastatic carcinoma cell lines from 3,2-dimethy1-4-aminobiphenyl-induced prostatic tumors in F344 rats." Jpn.J.Cancer Res.87(12). 1218-1226 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,K.et.al.: "Growth characteristics in the initial stage of micrometastasis formation by bacterial lacZ gene-tagged rat prostatic adenocarcinoma cells." Jpn.J.Cancer Res.87(12). 1227-1234 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujimitsu,Y.et.al.: "Development of aberrant crypt foci involves a fission mechanism as revealed by isolation of aberrant crypts." Jpn.J.Cancer Res.87(12). 1199-1203 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,K.et.el.: "Sequential observation of micrometastasis formation by bacterial lacZ gene-tagged Lewis lung carcinoma cells." Cencer Lett.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakanishi,H.et.al.: "Detection of tumor cells in blood and peritoneal lavage fluid of colorectal and gastric cancer patients by nested primer RT-PCR analysis. in "Recent advances in gastroenterological carcinogenesis" ed.E.Tahara." Monduzzi Editore.Bologna. (Italy) ., 853-856 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatematsu, M. et al.: "Clonal analysis of glandular stomach carcinogenesis in C3H/HeN【tautomer】BALB/c chimeric mice treated with N-methyl-N-nitrosourea." Cancer Lett.83. 37-42 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakanishi, H. et al.: "Progression of hormone dependent adenocarcinoma cells to hormone-independent spindle carcinoma cells in vitro in a clonal spontaneous rat mamary tumor cell line." Cancer Res.15. 399-407 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Imai, T. et al.: "Expression of hepatocyte growth foactor and c-met mRNAs during rat chemically induced hepatocarcinogenesis." Carcinogenesis. 17. 19-24 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi