| Project/Area Number |
07670300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
TAKEO Kanji Research Center for Pathogenic Fungi and Microbial Toxicoses Chiba University Professor, 真菌医学研究センター, 教授 (10027097)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Reiko Research Center for Pathogenic Fungi and Microbial Toxicoses Research Associate, 真菌医学研究センター, 助手 (60143319)
NISHIMURA Kazuko Research Center for Pathogenic Fungi and Microbial Toxicoses Chiba University Pr, 真菌医学研究センター, 教授 (00114314)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | Exophiala dermatitidis / ploidy / haploid / black yeasts / pathogenic fungi / diploid / DNA stain / nucleus / Capronia / 有性生殖 / 多形性黒色酵母 / DNA / 蛍光染色 / 定量測光 / Cryptococcus neoformans / 細胞周期 / DNA量 / ploidy / 有性性殖 / 無性性殖 |
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| Research Abstract |
A pathogenic black yeast Exophiala dematitidis was pleomorphic, and took yeast, pseudomycelium, mycelium, multicellular bodies, and two types of conidia. After fixation with 70% ethanol at 4ﾟC,cells (5-10mg) were suspended in test tube in 1 m1 of NS buffer supplemented with 5 mug/ml propidium iodide and lmg/ml RNase. The intensity of the fluorescence was measured with an Olympus BHS-RFC epifluorescence microscope equipped with a light intensity detector Olympus OSP-1.
Yeast cells were easiest to stain nuclear DNA quantitatively, followed by yeast-like pseudomycelia. These cells were obtained in most strains examined after incubation with shaking for one day in glucose, yeast extract and polypeptone medium (1% each) at 25ﾟC or 30ﾟC.
Most strains (53) of E.dermatitidis examined revealed similar sizes of the cells and of nuclei, and the amounts of DNA per cell were usual (unit amount). They were concluded as haploid. Three strains, however, exhibited clearly larger size of cells and nuclei and double amount of DNA per cell compared with usual strains. Thus, they were concluded as diploid.
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