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Research on biochemical analysis of mechanism of hemolysis induced by TDH produced by Vibrio parahaemolyticus.

Research Project

Project/Area Number 07670309
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

YOH Myonsun  Biken Osaka Univ.Res.Assoc., 微生物病研究所, 助手 (70093482)

Co-Investigator(Kenkyū-buntansha) IIDA Tetsuya  Biken.Osaka Univ.Res.Assoc., 微生物病研究所, 助手 (90221746)
HONDA Takeshi  Biken.Osaka Univ.Prof., 微生物病研究所, 教授 (60029808)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsVibrio parahaemolyticus / TDH / Biochemical study / Pathogenesis / Hemolysis / Phosphorylation
Research Abstract

We performed this study to make clear the mechanism of hemolysis induced by Vibrio parahaemolyticus thermostable direct hemolysin (TDH). Following results were obtained.
(1) TDH bound to erythrocytes both of which sensitive and insensitive to TDH in similar affinity. Binding of TDH to erythrocytes depends on temperature.
(2) TDH induced phosphorylation of 25 kDa protein on TDH-sensitive erythrocyte membrane but not on TDH-insensitive erythrocyte membrane.
(3) Phosphorylation of 25 kDa protein was inhibited by protein kinase C inhibitors. Hemolytic activity of TDH was also inhibited by protein kinase C inhibitors.
(4) Mutant TDH,R7 which could bind to erythrocytes but lost hemolytic activity, could not induce phosphorylation of 25 kDa protein.
These results suggest that the phosphorylation of 25 kDa protein on erythrocyte membrane is essential to lead erythrocytes lysis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yoh, M.: "Phosphorylation of a 25 kDa protein is induced by thermostable direct hemolysin of Vibrio parahaemolyticus." Int. J. Biochem. Cell. Biol.28. 1365-1369 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 余明順: "腸炎ビブリオ感染症" 化学療法の領域. 12. 633-640 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoh, M., N.Morinaga, M.Noda, and T.Honda: "The binding of Vibrio parahaemolyticus ^<125>I-labeled thermostable direct hemolysin to erythrocytes" Toxicon.33. 651-657 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoh, M., G.Q,Tang, T.Iida, N.Morinaga, M.Noda, and T.Honda: "Phosphorylation of a 25kDa protein is induced by thermostable direct hemolysin of Vibrio parahaemolyticus" Int.J.Biochem.Cell.Biol.23. 1365-1369 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoh, M., N.Kawakami, Y.Funakoshi, K.Okada, and T.Honda: "Evaluation of two assay kits for thermostable direct hemolysin (TDH) as an indicator of TDH-related hemolysin (TRH) produced by Vibrio parahaemolyticus" Microbiol.Immunol.39. 157-159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tang, G.Q., T.Iida, K.Yamamoto, and T.Honda: "Ca^<2+>-independent cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) on intestine 407, a cell line derived from human embryonic intestine" FEMS Micobiol.Lett.134. 233-238 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iida, T., G.Q.Tang, S.Suttikulpitug, K.Yamamoto, T.Miwatani, and T.Honda: "Isolation of mutant of Vibrio parahaemolyticus hemolysin by in vitro mutagenesis" Toxicon. 33. 209-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda, T., T.Miwatani, Y.Yabushita, N.Koike, and K.Okada: "A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayama, K., T.Oguchi, M.Arita, and T.Honda: "Purification and characterization of a cell-associated hemagglutination of Vibrio parahaemolyticus" Infect.Immun.63. 1987-1992 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayama, K., K.Yamamoto, T.Miwatani and T.Honda: "Characterization of a haemolysin related to Vp-TDH produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus" J.Med.Microbiol.42. 83-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoh,M.: "Phosphorylation of a 25 kDa protein is induced by thermostable direct hemolysin of Vibrio parahaemolyticus.21GC01:Int.J.Biochem.Cell.Biol." 28. 1365-1369 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 余 明順: "腸炎ビブリオ感染症" 化学療法の領域. 12. 633-640 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoh M.: "The binding of Vibrio parahaemolyticus 125I-labeled thermostable direct hemolysin to erythrocytes" Toxicon. 33. 651-657 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tang G.: "Ca-independent cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) on intestine 407,a cell line derived from human embryonic intestine" FEMS Microbiol.Lett.134. 233-238 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iida T.: "Isolation of mutant toxins of Vibrio parahaemolyticus hemolysin b in vitro nutage" Toxicon. 33. 209-216 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoh M.: "Evaluation of two assay kits for thermostable direct hemolysin (TDH) as an indicator of TDH-related hemolysin (TRH) produced by Vibrio parahaemolyticus" Microbiol.Immunol.39. 157-159 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tang G.: "Amutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes" Infect.Immun. 62. 3299-3304 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Xu M: "Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related homolysin gene (trh)" J.Bact.176. 4757-4760 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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