| Project/Area Number |
07670351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | TOKAI UNIVERSITY |
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Principal Investigator |
TAKEKOSHI Masataka TOKAI UNIV.SCH.OF MED.LECTURE, 医学部, 講師 (80221373)
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| Co-Investigator(Kenkyū-buntansha) |
IHARA Seiji TOKAI UNIV.SCH.OF MED.ASSISTANT PROFESSOR, 医学部, 助教授 (50096202)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | RECOMBINANT VIRUS / HUMAN CYTOMEGALOVIRUS / VACCINE / EXPRESSION VECTOR / HCMV |
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| Research Abstract |
We have tried to use human cytomegalovirus (HCMV) as a vector for gene therapy. It need to get HCMV that can't growth in the normal cells in safety. Then we have attempted to make a recombinant virus that essential gene, immediate early 1 (IE1), was deleted. First of all, we have constructed a cell line HIE1 expressed IE1 gene. An immortalized HEL cells were used as a parental cell line. Mutant generated by recombination in HIE1 between HCMV DNA and plasmid DNA which contained exon 4 deleted IE1 gene. Recombinant VMIE1 was plaque purified 3 times.
VMIE1 was infected normal HEL cells at a MOI of 2, then yielded progeny at a similar levels to wild type. In conclusion, IE1 deleted mutant is not sufficient for the vector for gene therapy.
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