| Project/Area Number |
07670367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
HATTORI Masakazu Kyoto University, Faculty of Medicine, Dept.of Immunology and Cell Biology, Assistant Researcher, 医学研究科, 助手 (40211479)
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| Co-Investigator(Kenkyū-buntansha) |
MINATO Nagahiro Kyoto University, Faculty of Medicine, Dept.of Immunology and Cell Biology, Prof, 医学研究科, 教授 (40137716)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Spa-1 / Rap1 / GAP / cell cycle progression / 11q13.3 / Rap1 / 11q13.3 / リンパ球活性化 / 低分子G蛋白 / 核内蛋白 / プロセッシング / GTPase活性化蛋白 / ロイシンジッパー / がん抑制遺伝子 |
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| Research Abstract |
We have cloned a human homologue of mouse Spa-1 gene (SPA-1) which encodes a 130 kDa (p130) cytoplasmic protein with SH3-binding, Rap1GAP.PEST and leucine zipper/coiled coil domains. p130 expressed in a Baculovirus system exhibits a specfic GTPase-activating activity against Rap1. Overexpression of a truncated Spa-1cDNA in NIH3T3 cells apparently disturbed the entry into the cell cycle in the G0-arrested NIH3T3 cells following mitogenic stimulation, suggesting that the protein is critically involved in the cell cycle regulation via the interaction with Rap1. Genomic mapping indicated that Spa-1 is located at the most centromeric region of chromosome 19 in mouse and at the centrimieric neighborhood of CCND1/PRAD1, a presumed BCL1 oncogene, at 11q13.3 in human.
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