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Induction of immune response against MHC class I-binding peptides

Research Project

Project/Area Number 07670372
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionOkayama University

Principal Investigator

NAKAYAMA Eiichi  Okayama University Medical School, Professor, 医学部, 教授 (60180428)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsMHC clas I / peptide / CTL / CD4 / APC / MAP (multiple antigen peptide) / 細胞傷害性T細胞(CTL) / 多価抗原 / MHC / 細胞傷害性T細胞
Research Abstract

1. Requirement of CD4 T cells for generation of specific CTL on stimulation with MHC class I-binding peptide.
We investigated generation of CTL in spleen cells from BALB/C mice on stimulation with p2Ca and pRL1 peptides. We found that CD4 T cells are necessary for generation of CTL in spleen cells against those peptides even though they are H-2L^d-binding peptides. Antigen presenting cells (APC) has also been shown to be necessary.
2. Induction of efficient CTL generation by pRL1peptide bound to MAP.
We investigated the effect of in vivo and in vitro stimulation with pRL1 peptide bound to MAP on induction of CTL generation. Stimulation with pRL1 peptide alone did not induce CTL generation, but stimulation with pRL1 peptide bound to MAP induced CTL generation. Cellular mechanisms of induction of CTL generaiton by the peptide bound to MAP should be further investigated.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Okumura,C.,et al.: "Induction of murine γδ T cells cytotoxic for xenogeneic rat cells." J.Immunol.154. 1114-1123 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,H.,et al.: "Requirement of CD4^+ T-cells and antigen presenting cells for primary in vitro generation of CD8^+ cytotoxic T-cells against L^d-binding self peptide p2Ca." Immunology. 84. 633-637 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Harutsumi,M.,et al.: "Rejection of parental Meth A tumor on concomitant inoculation of Meth A cells infecte retrovivally with the interferon-γ gene into (BALB/c x C57BL/6) F_1 mice." Int.J.Oncol.55. 4780-4783 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,H.,et al.: "Rejection antigen peptides on BALB/c RL ♂ 1 leukemia recognized by cytotoxic T lymphocytes : derivation from the normally untranslated 5′-region of the c-akt proto-oncogene activated by LTR." Cancer Research. 7. 233-238 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ono,T.,et al.: "Production of murine leukemia RL ♂ 1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b." Jpn.J.Cancer Res.87. 1165-1170 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okumura, C., et al.: "Induction of murine gammadelta T cells cytotoxic for xenogeneic rat cells." J.Immunol.154. 1114-1123 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada, H., et al.: "Requirement of CD4^+ T-cells and antigen presenting cells for primary in vitro generation of CD8^+ cytotoxic T-cells against L^d-binding self peptide p2Ca." Immunology. 84. 633-637 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Harutsumi, M., et al.: "Rejection of parental Meth A tumor on concomitant inoculation of Meth A cells infected retrovivally with the interferon-gamma gene into (BALB/c x C57BL/6) F_1 mice." Int.J.Oncol.7. 233-238 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada, H., et al.: "Rejection antigen peptides on BALB/c RL*1 leukemia recognized by cytotoxic T lymphocytes : derivation from the normally untranslated 5'-region of the c-akt proto-oncogene activated by LTR." Cancer Research. 55. 4780-4783 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ono, T., et al.: "Production of murine leukemia RL*1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b." Jpn.J.Cancer Res.87. 1165-1170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,H.,et al.: "Rejection antigen peptides on BALB/cRL♂1 leukemia recognized by cytotoxic T lymphocyles : derivation from the normally untranslated 5'-region of the c-akt proto-oncogen activated by LTR." Cancer Research. 55. 4780-4783 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chen,B.-G.,et al.: "Inhibition by CsA and FK506 of the in vitro proliferative response of γδT cells on stimulation with anti-TCR δ mAb." Transplant Immunol.4. 158-162 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ono.T.,et al.: "Production of murine leukemia RL♂1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b." Jpn.J.Cancer Res.87. 1165-1170 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kakeya,H.,et al.: "A 77 kDa protein from cryptococcus neoformans,a member of the heat shock protein 70 family,is a major antigen detected by sera of mice with pulmonary cryptococcosis." Infect.Immunity. (in press.).

    • Related Report
      1996 Annual Research Report
  • [Publications] 中山睿一: "細胞傷害性T細胞の認識する腫瘍抗原ペプチド" Molecular Medicine臨時増刊号《免疫1996-97》. 33. 92-100 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中山睿一: "腫瘍抗原ペプチド" Mebio. 13. 44-49 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okumura,C.,et al.: "Induction of murine γδ T cells cytotoxic for xenogeneic rat cells." J.Immunol.154. 114-1123 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,H.,et al.: "Requirement of CD4^+ T-cells and antigen presenting cells for primary in vitro generation of CD8^+ cytotoxic T-cells against L^d-binding self peptide p2Ca." Immunology. 84. 633-637 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsutake,T.,et al.: "Prevention of mortality by in vivo depletion of αβ T cells in murine lethal listeriosis and involvement of γδ T cells in bacterial elimination." Immunobiology. 193. 71-83 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harutsumi,M.,et al.: "Rejection of parental Meth A tumor on concomitant inoculation of Meth A cells infected retrovirally with the interferon-γ gene into (BALB/c x C57BL/6) F_1 mice." Int.J.Oncol.7. 233-238 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Baba,H.,et al.: "Two isotypes of murine nm23/nucleoside diphosphate kinase,nm23-M11 and nm23-M2 are involved in metastatic suppression of a murine melanoma line." Cancer Research. 55. 1977-1981 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,H.,et al.: "Rejection antigen peptides on BALB/c RL♂1 leukemia recognized by cytotoxic T lymphocytes : derivation from the normally untranslated 5'-region of the c-akt proto-oncogene activated by LTR." Cancer Research. 55. 4780-4783 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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