| Project/Area Number |
07670418
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH |
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Principal Investigator |
KASE Tetsuo OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH SENIOR RESEARCHER, 公衆衛生部, 主任研究員 (10175276)
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| Co-Investigator(Kenkyū-buntansha) |
OKUNO Yoshinobu OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH SECTION CHIEF, 公衆衛生部, ウイルス課長 (30112064)
MAEDA Akiko OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH SENIOR RESEARCHER, 公衆衛生部, 主任研究員 (40250279)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Adenoviruses / Hexon / PCR / Nucleotide sequence / Endonuclease analysis / Identification / A rapid diagnosis / シークエンス |
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| Research Abstract |
Adenoviruses cause a pharyngoconjunctival fever, an epidemic keratoconjunctivitis, an acute hemorrhagic cystitis, an infant diarrhea, an infant pneumonia and so on. In ophthalmology and pediatrics they need a rapid diagnosis for adenoviral infection especially, as adenovirus may become an agent of nosocomial infection disease. Therefore, the applicability of polymerase chain reaction (PCR) assay in diagnosing adenoviral infection was studied. We could obtain the PCR products from 1-8 serotype of adenovirus using a primers set for the hexon coding region. The each PCR products was sequenced and analyzed to get restriction enzyme sites. In consequence all sequences of PCR products were not identical and each of them had different restriction sites. We showed here that an endonuclease analysis of the PCR products by electrophoresis in the agarose gel after treatment with Hae III,Hpa II,Rsa I and Mae I might identify adenoviruses. Eventually two PCR products from eye swab in which adenovirus was not isolated had the same endonuclease analysis patterns with the PCR products of specimen recovered adenoviruse type 8 in the nosocomial infection. The two patients not recovered viruses also seemed to infect with adenovirus type 8. These results suggest that this endonuclease analysis could be useful in diagnosing adenoviral infection, even when viruses were not isolated in tissue culture.
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