| Project/Area Number |
07670419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
YAMAYOSHI Takao Osaka Prefectural Institute of Public Health, Department of Public Health, Chief Researcher, 公衆衛生部, 主任研究員 (70231678)
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| Co-Investigator(Kenkyū-buntansha) |
KUMEDA Yuko Osaka Prefectural Institute of Public Health, Department of Public Health, Depar, 食品衛生部, 主任研究員 (10250317)
TANAKA Hidetsugu Osaka Prefectural Institute of Public Health, Cheif Researcher Osaka Prefectural, 公衆衛生部, 主任研究員 (10250292)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | ATP Luminescence / Evaluation of cytotoxicity / chemical contaminants in environments / Leciferin-Luciferae / ATPLuminescence |
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| Research Abstract |
Pollutants of the living environment are known to pass through various biodefensive barriers of cells and cause cytotoxicity and to be metabolized, resulting in toxicity and carcinogenicity. Therefore, a method to evaluate the effects of chemicals with carcinogenicity but low mutation induction rates in short-term assay was investigated using ATP as an endpoint.
MOLT-4 cells, a helper T-cell derived cell line, were used in the study. Pentachlorophenol (PCP) and phenol were used as chemicals with a low mutation induction rate. As a result, it was evident that the cytotoxic concentration fifty (CC_<50>) of PCP was 50-fold that of phenol when CC_<50> was used to express the toxicity of PCP and phenol on MOLT-4 cells. Even though PCP did not induce any genetic mutatins and no mutagenicity was observed in short-term assay, the toxicity of PCP was found to be high, 50 times that of phenol.
From these results, it appears that this is a rapid, high precision effective method which can be used in the cytotoxicity evaluation of chemicals which do not induce genetic mutants.
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