| Project/Area Number |
07670494
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Kagawa Medical University |
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Principal Investigator |
IJIRI Iwao Kagawa Medical University・Medicine Professor, 医学部, 教授 (90113183)
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| Co-Investigator(Kenkyū-buntansha) |
FUKE Chiaki University of the Ryukyus・Medicine Assosiate Professor, 医学部, 助教授 (60173374)
KINOSHITA Hiroshi Kagawa Medical University・Medicine Assistant, 医学部, 助手 (00284357)
AMENO Kiyoshi Kagawa Medical University・Medicine Assosiate Professor, 医学部, 助教授 (50019626)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Ethanol / Acetaldehyde / Intestinal Absorption / Histamine / In Situ Single-Pass Perfusion Technique / Intestinal Blood Flow / マイクロスフェア-法 |
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| Research Abstract |
We have studied the effects of high blood acetaldehyde concentrations on the intestinal absorption of ethanol using an in situ single-pass perfusion technique on the rat intestine. We found that high blood acetaldehyde concentrations inhibit intestinal ethanol absorption in an inverse proportion to peak acetaldehyde concentrations, decrease intestinal blood flow, and inhibit intestinal absorption of 2-butanone. The decrease of the intestinal blood flow, induced by high blood acetaldehyde concentrations, is a major mechanism inhibiting intestinal ethanol absorption, but other mechanisms are also thought to inhibit absorption.
We have observed an increase in volume of collected fluid after perfusionin a state of high blood acetaldehyde concentration. Since secretion from the intestinal glands increases, we tried to study the changes of histamine and prostaglandinF_2 after perfusion. Histamine in perfused fluid increased after pretreatment with cyanamide, but prostaglandinF_2 tended to increase after the pretreatment. We couldn't evaluate the results under various datadue to ambiguity.
Therefore, the changes of histamine and catecholamine in intestinal tissue after perfusion will be studied instead.
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