| Project/Area Number |
07670495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nagasaki University School of Medicine |
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Principal Investigator |
KUBO Shin-ichi Nagasaki Univ.Sch.of Med., Dep.Legal Med., Associate Prof., 医学部, 助教授 (10205122)
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| Co-Investigator(Kenkyū-buntansha) |
TSUDA Ryouichi Nagasaki Univ.Sch.of Med., Dep.Legal Med., Asistant Prof., 医学部, 講師 (20098875)
NAKASONO Ichiro Nagasaki Univ.Sch.of Med., Dep.Legal Med., Professor., 医学部, 教授 (30108287)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | DNA profiling / PCR / personal identification / organized bone marrows / sex determination |
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| Research Abstract |
We examined the organized human bone marrows with the aim of personal identification using PCR (the Polymerase Chain Reaction) analysis, that is sex determination and STR (short tandem repeats). The purpose of the present research is to disclose the optimum device to detection of DNA polymorphism with degraded DNA samples. The subjects were 38 manubrium sterni sampled at autopsies. We left them outdoor for periods from two weeks to 36 months, then DNA samples were extracted from bone marrows.
1.We compared two primers of different bp length from amelogenin gene for sex determination. The sex identifications in 18 subjects were determinable with longer bp AMGL at first PCR analysis. Regarding to the other sex undeterminable cases, even then ten cases have still **en undeterminable although we took low molecules away and added BSA at PCR and moreover we tested Dual PCR.On the contrary, 25 ***aples were sex determinable with shorter bp AMGL at first PCR analysis. All cases have been sex de
… More
terminable when we performed PCRs when we removed low molecules form samples and added BSA.Thus we have arrived at the conclusion that followings are necessary to determine sexes with degraded DNA samples. Those are removal of low molecules and addition of BSA to the DNA samples Then designing shorter bp primer is preferable.
2.We investigated STR types with vWF and TH01. Then we assessed the results whether or not those results would coincide between the samples providing from organized bone marrows and the blood DNA samples from each of the same individuals at autopsies. Thirteen samples were not amplified without preparation of DNA just the same as sex determination, but the products of all cases were clearly visible following the above preparation. The bands from organized bone marrow DNA and blood DNA at the time of autopsy coincided in all cases. Since both vWF and TH01 are shorter primers, when personal identification is demonstrated from organized DNA,it is necessary to increase the sensitivity of detection regarding to both DNA preparation and designing of the primers. Less
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