| Project/Area Number |
07670514
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Chiba University School of Medicine |
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Principal Investigator |
KOHNO Yoichi Chiba Univ Sch Med, Associate Professor, 医学部, 助教授 (60161882)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Bronchial asthma / Outgrow / House dust mite / T cell line / Cytokine / Flow cytometry / Hyposensitization / インターロイキン-4 / インターロイキン-5 / インターフェロン-γ |
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| Research Abstract |
In order to investigate the mechanisms of outgrow of bronchial asthma, we studied (1) cytokine production of house dust mite allergen-stimulated T cells either in patients with active asthma or in patients who outgrew from asthma by newly developed system for detection of intracellularly produced cytokine by use of flow cytometry, (2) cytokine production of house dust mite-specific T cell lines established from peripheral blood mononuclear cells before and after allergen-specific immunotherapy. Flow cytometrical analysis revealed that peripheral CD4_+ T lymphocytes in active asthma patients stimulated with house dust mite allergen produced much higher amounts of interleukin 4 (IL-4) and IL-5 than those in outgrown patients. House dust mite-specific T cell lines established before immunotherapy produced greater amounts of IL-4 and IL-5 than those established from the same patients after immunotherapy. These results indicate that reduction of Th2 cytokines such as IL-4 and IL-5 from house dust mite-reactive T cells may be related to outgrow of bronchial asthma both in spontaneous and therapy-based outgrow.
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