| Project/Area Number |
07670516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Chiba University |
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Principal Investigator |
NAWATA Yasushi Chiba University School of Medicine, Assistant, 医学部, 助手 (60228103)
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| Co-Investigator(Kenkyū-buntansha) |
KURASAWA Kazuhiro Chiba University School of Medicine Assistant, 医学部, 助手 (30282479)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Dermatomyositis / Immune response / Growth factor / Cytokine |
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| Research Abstract |
Dermatomyositis (DM) and polymyositis (PM) are classified into the idiopathic inflammatory myopathy. The pathoetiology of these diseases has not been clarified although autoimmune mechanism has been postulated. Recently, the difference in the pathogenesis of myositis has been indicated between PM and DM.DM has especially poor prognosis because of the frequent complication of malignancy and severe interstitial pneumonitis. To determine whether growth factors and/or cytokines are involved in the pathogenesis of DM,we evaluated their localization in the muscle. It was revealed that b-FGF was strongly expressed at the surface and the nuclei of muscle fibers with stronger expression in PM especially those with high CPK levels, suggesting its relation with muscular damage and regeneration in PM.TGF-beta was localized at endomysium and endomysial infiltrating cells in PM,and was remarkable at the perivascular infiltrating cells of perimysium in DM.The activation of TGF-beta at the site of cell infiltration was suggested. PDGF-AA and PDGF-BB showed no specific localization. IFN-gamma was found at the surface of muscle fibers near the infiltrating cells, perivascular infiltrating cells, and endothelium both in PM/DM.TNF-alpha and IL-1beta were not observed at muscle fibers in spite of the expression at infiltrating cells. ICAM-1 was observed at infiltrating cells and adjacent muscle fibers in PM,and its expression at endothelial cells was only observed in DM.These results suggest that the immune response at perimysial perivascular infiltrating cells and endothelial cells is important in the pathogenesis of DM.Although the participation of growth factors in DM was not shown, b-FGF and TGF-beta were considered to have some role in myositis.
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