| Project/Area Number |
07670566
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
SHIINA Shuichiro University of Tokyo, Department of Medicine (Hospital) Instructor, 医学部(病), 助手 (70251238)
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| Co-Investigator(Kenkyū-buntansha) |
OMATA Masao University of Tokyo Department of Medicine (Hospital) Professor & Chairman, 医学部(病), 教授 (90125914)
KANAI Fumihiko Nihon Gakujyutsu Shinkokai, Exective Reseach Fellow, 特別研究員
KATO Naoya University of Tokyo Department of Medicine (Hospital) Fellow, 医学部(病), 医員
MATSUMURA Masayuki University of Tokyo Department of Medicine (Hospital) Fellow, 医学部(病), 医員
NIWA Yasuro University of Tokyo Department of Medicine (Hospital) Instructor, 医学部(病), 助手
丹羽 泰郎 東京大学, 医学部・附属病院, 医員
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | hepatocellular carcinoma / nucleic acid sequence / AFP / AFP gene / transcriptional factor / 肝細胞癌 / αFP / 癌化 / αFP遺伝子 / 生検材料 / 遺伝子発現調節機構 |
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| Research Abstract |
We have performed ultrasound-guided percutaneous fine needle biopsy on approximately 150 cases of small hepatocellular carcinoma and have reserved the specimens in liquid nitrogen. A part of each specimen has been reserved with isogen in order to abstract RNA.We have also obtained samples of non-cancerous liver tissue as the control from each cases.
We expect that comparison of nucleic acid sequence between the cancerous tissue and the non-cancerous tissue of the same patient will make it clear whether the carcinogenesis has produced any change in the nucleic acid sequence. Thus, the reserved samples were handled with protenase K,DNA was abstracted by the phenol-chroloform method, and the obtained DNA was amplified by PCR using primers set in the promoter and enhancer of AFP gene. Then we tried to determine the nucleic acid sequence of each sample by a sequencer, but we have not had reproducible results yet.
We also homogenated the reserved sample by Dounce homogenizer, and centrifuged the homogenate and precipitated the nucleic fragment. Then we homogenated the precipitate in a buffer again, dialyzed it in the buffer, removed unsolvable materials, and obtained the nucleic abstract. Then we tried to examine the presence and quantity difference of transcriptional factors, but we have not found a certain trend yet.
We will continue the experiment until we obtain stable results.
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