Analysis of beta-catenin gene in gastric cancer cells
| Project/Area Number |
07670605
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | SAPPORO MEDICAL UNIVERSITY |
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Principal Investigator |
KATO Junji (1996) SAPPORO MEDICAL UNIVERSITY,assistant professor., 医学部, 講師 (20244345)
高橋 康雄 (1995) 札幌医科大学, 医学部, 助手 (10236325)
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| Co-Investigator(Kenkyū-buntansha) |
加藤 純二 札幌医科大学, 医学部, 講師 (20244345)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | gastric cancer / adhesion / cadherin / catenin / cell cycle / cdk inhibitor / p21 / p27 / E-cadherin / β-catenin / スキルス胃癌 / adherence junction / transfection / Southern blot |
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| Research Abstract |
Detachment of cell-cell adhesion is indispensable for the first step of invasion and metastasis of cancer cells. We have recently demonstrated that the function of E-cadherin was completely abolished in HSC-39, despite the high expression of E-cadherin, because of mutations in one of the E-cadherin-associated cytoplasmic proteins ; beta-catenin. In the present study, we firstly investigated beta-catenin gene expression in various human gastric cancer cells. It was revealed that a gastric carcinoma cell line KATO-III expressed the rearranged beta-catenin gene with amplification. Recently, it has been reported that beta-catenin binds to both APC and ErbB-2. Thus the function of E-cadherin system is supposed to affect the cell proliferation as well as cell-cell adhesion. We then transfected a wild-type beta-catenin gene expression vector into HSC-39 cells to examine the beta-catenin-mediated signal transduction. E-cadherin dependent cell-cell adhesiveness was recovered by the transfection of wild-type beta-catenin cDNA as revealed by cell compaction, cell-aggregation and immunofluorescence staining. In HSC-39beta cells, the cell proliferation, anchorageindependent growth and tumorigenecity were significantly reduced as comared with those of parental cells. Furthermore, the expressions of p21^<Cip1> and p27^<Kip1>, which inhibit the progression of cell cycle from G1 to S phase, were remarkably increased in HSC-39/beta. These results suggested that beta-catenin is involved in the cell cycle regulation as well as E-cadherin dependent cell-cell adhesion system.
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Report
(3 results)
Research Products
(18 results)
