| Project/Area Number |
07670617
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical College |
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Principal Investigator |
SAITO Toshihiko Tokyo Medical College, 4 th department of internal medicine, Professor, 医学部, 教授 (80074582)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Yumiko Tokyo Medical College 4th department of internal medicine, assistant, 医学部, 助手 (90246257)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Oncogene / Stool / Colorectal cencer / K-ras / Mutant-allele-specific-amplification / Analysis of genetic alteration / ras遺伝子 / mutant allele specific amplification |
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| Research Abstract |
Using mutant-allele-specific amplification procedure, patients with colorectal tumors were analyzed for K-ras point mutations in the stool and the tumor tissue.
K-ras mutation of DNA purified from the stool was detected in 10 of 40 (25.0%) cancer patients, and in 3 of 10 (30.0%) adenoma patients. Otherwise, in the case whose tumors contained the mutations it was detected with the frequency of 71.4% in cancers, and 100% in adenomas. This frequency tended to decrease in the cancers of distal colon or small size but there was no significant.
This study suggested that stool analysis of genetic alterations would develop diagnostic method for colorectal cancer.
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