| Project/Area Number |
07670621
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
SHIRATORI Keiko Tokyo Women's Medical College, Department of Medicine, Associatet Professor, 医学部, 助教授 (70101855)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | secretin / cholecystokinin (CCK) / CCK receptor antagonist / gastrin / pancreatic secretion / gastric acid secretion / pancreatic islet / インスリン / CCK受容体 / 脂肪 / 胃液分泌 / 膵液分泌 / ガストリン / リパーゼ |
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| Research Abstract |
It has been well known that pancreatic enzymes have a most important role in the intestinal digestion, and gastric and pancreatic secretion is regulated by digestive products. We investigated the regulation of gastric and pancreatic secretion in response to intestinal nutrients (protein and fat) mediated by gastrointestinal hormones in rats.
1.Effect of intraduodenal fat (fat, lipase-digested fat, and fatty acid) on gastric acid secretion and gastrin release was studied. Although lipase-digested fat and fatty acid inhibited acid secretion and gastrin release, fat failed the inhibitory effect under diversion of pancreatic juice. It means that fat digestion by lipase is necessary for the inhibitory action of intraduodenal fat on gastric acid secretion.
2.Pancreatic stimulatory effect of intraduodenal long-and medium-chain trigyceride (LCT and MCT) was studied. Pancreatic secretion and CCK release was stimulated by LCT under pancreatic juice existed in the duodenum, but not affected by MCT.Under pancreatic juice diversion, LCT failed to stimulate pancreatic secretion. Thus, LCT digestion by pancreatic juice is necessary for stimulating pancreatic secretion.
3.Effect of intraduodenal protein (casein) and amino acids on pancreatic secretion and release of secretin and CCK was studied. Casein protein stimulated CCK release and enzyme secretion. On the other hand, amino acids stimulated secretin release and bicarbonate secretion. Thus, protein digestion affects hormone release and pancreatic secretion.
4.Intraduodenal administration of amino acids as well as fat, inhibited gastric acid secretion and gastrin release. The inhibitory action of amino acids was mediated by endogenous secretin released by amino acids.
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