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STUDY OF EXPRESSION IF VEGF AND IT'S RECEPTOR

Research Project

Project/Area Number 07670627
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

KAWAHARA Hiromu  KANAZAWA MEDICAL UNIVERSITY,MEDICINE,ASOCIATED PROFESSOR, 医学部, 講師 (10177727)

Co-Investigator(Kenkyū-buntansha) SAWADA Makoto  KANAZAWA MEDICAL UNIVERSITY,MEDICINE,ASSISTANT, 医学部, 助手 (50170824)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsVEGF / Hepatocellural carcinoma / Necrosis / Metastasis / 血管新生 / Hyoxia
Research Abstract

Expression of VEGF was studied in human hepatoma derived cell line (HepG2) and human hepatoma tissues. VEGF is known of four subtypes of messenger RNA, due to splicing sites. Three subtypes of VEGF, VEGF_<121>, VEGF_<165> and VEGF_<189>, were expressed, by HepG2 cells using RT-PCR with a set of primers detecting whole length mRNA from exoni to exon8. In non-regenerative nodules, hepatocytes were stained weakly by immunohistochemistry with VEGF antibody. Smooth muscle cells of small vessels and duct epithelial cells in the fibrous stroma were also positive for VEGF.In human hepatoma, hepatoma cells, especially in periphery of necrotic parts, were stained markedly. In addition, hepatoma cells invading the small vessels of fibrous stroma were also strongly positive for VEGF stain. These results indicated that hepatoma cells in vitro and in vivo expressed VEGF supporting neovascularization and metastasis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 川原 弘: "肝癌の転移・増殖とその分子機構:肝細胞癌の浸潤転移における血管増生因子(VEGF)の意義" 肝胆膵. 34. 207-213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawahara H.: "Messenger RNA expression of vascular endothelial growth factor(VEGF)in human hepatoma-derivred cell line(abstract)" Int Hepatol Commun. 3(Suppl.). S149 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawahara H: "Messenger RNA expression of vascular endothelial growth factor (VEGF) in human hepatoma-derived cell line" Int Hepatol Commun. 3(Suppl). S149 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawahara H.: "Messenger RNA expression of vascular endothelial growth factor(VEGF)in human hepatoma-derivred cell line(abstract)" Int Hepatol Commun. 3(Suppl. ). S149 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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