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Studies on the pathogenesis of early lesions in Crohn's disease : Clinical and experimental investigation

Research Project

Project/Area Number 07670633
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKAWASAKI MEDICAL SCHOOL

Principal Investigator

IIDA Mitsuo  KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (00127961)

Co-Investigator(Kenkyū-buntansha) KUROKI Fumitoshi  KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (20278956)
MATSUMOTO Takayuki  KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (10278955)
FUJIMURA Yoshinori  KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (30156905)
鴨井 隆一  川崎医科大学, 医学部, 講師 (60186020)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCrohn's disease / M cell / colonic lymphoid nodule / indomethacin / small intestinal ulcer / electron microscopy / proliferative activities of the mucosa / growth factor / HLA-DR / 大腸拡大内視鏡 / 初期病変
Research Abstract

1. Clinical investigation
To clarify the role of the follicle associated epithelium (FAE) of colonic lymphoid nodules in the formation of ulcers in Crohn's disease, 10 patients with this disease were studied by magnifying colonoscopy, electron microscopy, and immunohitochemical examination. As a result, scanning electron microscopy of lymphoid nodules surrounded by a red halo without visible erosions by magnifying colonoscopy showed surface erosions 150-120mum in size. Correlation of scanning and transmission electron microscopy revealed residues of FAE including M cells at the edges of the erosions. In immunohistochemical studies, HLA-DR antigen was more strongly expressed in the entire inflamed colonic mucosa in the active stage of Crohn's disease than in the remission stage. Therefore, the red halo appearance surrounding lymphoid follicles seems to preced visible aphthoid ulcers and suggests that ulcerations in Crohn's disease originate from FAE.
2. Experimental investigation
To charac … More teriza early changes in both cryptal proliferation and expression of growth factors in indomethacin-induced small intestinal damage, scanning electron microscopic findings, cryptal proliferation (using immunohistochemical staining for Brd U), and intestinal mRNA expression of TGF-beta_1, IGF-1, HGF,and NGF (using RT-PCR) were investigated in rats which were received 24 mg/kg of intracolonic indamethacin. Scanning electron microscopy showed that the villi in the mid-small intestine was distorted with tortuous fashion at an hour. Cryptal proliferation in the mid-small intestine was not altered at 2 hour whereas it was decreased at all sites after 5 hours. IGF-1 and HGF expression became obvious at 6 hour, while NGF could not be detected at this time point. These findings suggest that early mucosal damage induced by indomethacin is characterized by alteration of the crypt architecture without changes in crypt cell proliferation, and that changes of some growth factors may play a role in the progression of the mucosal damage. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Fujimura Y.: "Pathogenesis of aphthoid ulcers in Crohn's disease : correlative observations by magnifying colonoscopy,electron microscopy and immunohistochemistry." Gut. 38. 724-732 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 松本主之: "インドメサシン小腸潰瘍" Research Furum on Digestive Disease. 3. 97-102 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 藤村宜憲: "大腸M細胞の構造と機能-腸内抗原に対するセンサー細胞-" 細胞. 27. 129-134 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimura Y,et al.: "Pathogenesis of aphthoid ulcers in Crohn's disease : correlative findings by magnifying colonoscopy, electron microscopy, and immunohistochemistry" Gut. 38. 724-732 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto T,et al.: "Indomethacin-induced enteropathy" Research Forum on Digestive Disease. 3. 97-102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimura Y,et al.: "Structure and function on M cells in the large intestine." The Cell. 27. 129-134 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimura Y.: "Pathogenesis of aphthoid ulcers in Crohn's disease : correlative observations by magnifying colonoscopy,electron microscopy and immunohistochemistry." Gut. 38. 724-732 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松本主之: "インドメサシン小腸潰瘍" Research Forum on Digestive Disease. 3. 97-102 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 藤村宜憲: "大腸M細胞の構造と機能-腸内抗原に対するセンサー細胞-" 細胞. 27. 129-134 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujimura Y.: "Pathogenesis of aphthoid ulcers in Crohn's disease : correlative observations by magnifying colonoscopy, electron microscopy and immunohistochemistry." Gut. (accepted).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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