Studies on the pathogenesis of early lesions in Crohn's disease : Clinical and experimental investigation
| Project/Area Number |
07670633
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | KAWASAKI MEDICAL SCHOOL |
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Principal Investigator |
IIDA Mitsuo KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (00127961)
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| Co-Investigator(Kenkyū-buntansha) |
KUROKI Fumitoshi KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (20278956)
MATSUMOTO Takayuki KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (10278955)
FUJIMURA Yoshinori KAWASAKI MEDICAL SCHOOL・FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (30156905)
鴨井 隆一 川崎医科大学, 医学部, 講師 (60186020)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Crohn's disease / M cell / colonic lymphoid nodule / indomethacin / small intestinal ulcer / electron microscopy / proliferative activities of the mucosa / growth factor / HLA-DR / 大腸拡大内視鏡 / 初期病変 |
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| Research Abstract |
1. Clinical investigation
To clarify the role of the follicle associated epithelium (FAE) of colonic lymphoid nodules in the formation of ulcers in Crohn's disease, 10 patients with this disease were studied by magnifying colonoscopy, electron microscopy, and immunohitochemical examination. As a result, scanning electron microscopy of lymphoid nodules surrounded by a red halo without visible erosions by magnifying colonoscopy showed surface erosions 150-120mum in size. Correlation of scanning and transmission electron microscopy revealed residues of FAE including M cells at the edges of the erosions. In immunohistochemical studies, HLA-DR antigen was more strongly expressed in the entire inflamed colonic mucosa in the active stage of Crohn's disease than in the remission stage. Therefore, the red halo appearance surrounding lymphoid follicles seems to preced visible aphthoid ulcers and suggests that ulcerations in Crohn's disease originate from FAE.
2. Experimental investigation
To charac
… More
teriza early changes in both cryptal proliferation and expression of growth factors in indomethacin-induced small intestinal damage, scanning electron microscopic findings, cryptal proliferation (using immunohistochemical staining for Brd U), and intestinal mRNA expression of TGF-beta_1, IGF-1, HGF,and NGF (using RT-PCR) were investigated in rats which were received 24 mg/kg of intracolonic indamethacin. Scanning electron microscopy showed that the villi in the mid-small intestine was distorted with tortuous fashion at an hour. Cryptal proliferation in the mid-small intestine was not altered at 2 hour whereas it was decreased at all sites after 5 hours. IGF-1 and HGF expression became obvious at 6 hour, while NGF could not be detected at this time point. These findings suggest that early mucosal damage induced by indomethacin is characterized by alteration of the crypt architecture without changes in crypt cell proliferation, and that changes of some growth factors may play a role in the progression of the mucosal damage. Less
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Report
(3 results)
Research Products
(10 results)
