| Project/Area Number |
07670640
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
|
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| Research Institution | Fukui Medical School (1996)
Tokyo Metropolitan Organization for Medical Research (1995) |
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Principal Investigator |
YABU Koji Fukui Medical School, Assistant, 医学部, 助手 (60240793)
|
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| Co-Investigator(Kenkyū-buntansha) |
小原 道法 財団法人東京都臨床医学総合研究所, 微生物研究部門, 研究員 (10250218)
|
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| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Chronic hepatitis C / recombinant antigens of HCV / cellular immunological responses / antigen-spesific proliferative responses |
|---|
| Research Abstract |
The assumption is that the reason why patients with chronic hepatitis C can not eradicate hepatitis C virus (HCV) in natural course is that immunological defense mechanisms against HCV does not work enough in such patients. It is generally accepted that cellular immunological defense of the host play important roles in exclusion of the virus persistently infecting. It has not been fully examined in cellular immunological aspects to eradicate HCV.The purpose of this study is to investigate how T lymphocytes of the patients respond in vitro to HCV antigens derived from recombinant HCV proteins or glycoproteins. Recombinant antigens to the genetic area coding HCV core (Pc), HCV envelopes of E1 and E2/NS1 (Ac-E1 and Ac-NS-1) and HCV non coding region of NS3 (Pn3) were made by recombined baculovirus or E.coli. The lymphocyte proliferative responses to these recombinant HCV antigens were tested using lymphocytes separated from peripheral blood lymphocyte of the patients with CH-C.
NS-3 and core-antigen specific responses in all patients groups were significantly higher that in the healthy control groups. E-1 and E2/NS1-antigen-specific responses in the patients group with ALT levels exceeding 100 IU/L were significantly higher than those in other patient groups. Histological diagnosis was not correlated to the intensity of the core-and NS3-specific responses. E1-and E2/NS1-antigens induced significantly elevated responses in patients with chronic active hepatitis and liver cirrhosis compared with results in the healthy control group and in patients with chronic presistent hepatitis. In conclusion, the significantly elevated responses to core-and NS3-antigens may be related to HCV infection and such responses to E1-and E2/NS1-antigens could be related to the severity and activity of the disease.
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