| Project/Area Number |
07670642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
HIRAIWA Nozomu Aichi Cancer Center, Experimental Pathology, Senior Researcher, 研究所病理学第二部, 主任研究員 (70175553)
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| Co-Investigator(Kenkyū-buntansha) |
KANNAGI Reiji Aichi Cancer Center, Experimental Pathology, Chief, 研究所病理学第二部, 部長 (80161389)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | glycosyltransferase / sulfated glycolipid / expression cloning / hepatoma / HNK-1 / パンニング |
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| Research Abstract |
We have tried to isolate a cDNA clone encoding human sulfotransferase, which could synthesize sulfated LacCer. In this research, we used a mammalian expression system with monoclonal antibodies which were established against sulfated glycolipids in our laboratory, and found that de novo expression of transgected cells. However, we are still in the way to get a cDNA that represent a tructural gene encoding a human sulfotransferase.
At the same time, we have investigated expression pattern of the GalCer sulfotransferase which was recently cloned, among human liver and hepatoma cell lines. In Northern blots, the gene was expressed in human hepatoma cell lines in relation to product of sulfoglycolipids in these cells. This result might suggest that the enzyme encoded by the gene could synthesize sulfated LacCer, and this issue should be resolved upon analysis of enxymatic function derived from this gene.
Among acidic glycolipids and glycoproteins, sialylated glyco-structures have function of cell adhesion like sulfated glycolipids, and we have investigated that expression of glycosyltransferases related to these products were actually regulatted in cancer-related and cell-specific condition. Especially some transferase is under the influence of the transcription activator of the oncovirus in synthesis of sialyl Le^x which mediates adhesion of leukocytes and leukemic cells to endothelium.
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