| Project/Area Number |
07670644
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
MUNAKATA Mitsuru Hokkaido University, School of Medicine, First Department of Medicine, Lecturer, 医学部, 講師 (00209991)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HOMMA Yuhihiko Hokkaido University, School of Medicine, First Department of Medicine, Professor, 医学部, 教授 (30001902)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | Rronchial Asthma / Bronchial Hyperresponsiveness / Airway Remodeling / Rat / 気道リモデリング / 平滑筋増生 / ゴブレット細胞 |
|---|
| Research Abstract |
To elucidate the mechanisms of airway remodeling in bronchial asthma, chronic asthma model with repeated ovalbumine (OA) exposure was developed. By examining this model, following results were obtained. 1) In the protocol examining the effects of inhaled antigen concentration, rats were exposed to 0.1,1.0 and 5.0% OA every other day for 1 month. In this setting, airway epithelial and wall thickening were observed only in 1.0 and 5.0% OA exposure groups. This result suggest that the concentration of inhaled antigen is important in inducing airway remodeling. 2) In present rat asthma model, repeated exposuure to OA for 1 month resulted airway epithelial and wall remodeling but airway smooth muscle thickening was not observed. This results suggest that airway epithelial remodeling may proceed to smooth muscle remodeling. 3) Goblet cell hyperplasia was revealed to be one of the factors responsible for airway epithelial thickening. 4) There was significant negative correlation between the number of goblet cells in the epithelium and in vivo methacholine airway responsiveness. 5) In the protocol examining the effects of continuous infusion of beta2-agonist and corticosteroid by osmotic pomp, enhanced airway responsiveness to methacholine was observed after beta2-agonist infusion. However, there was no significant effects of these drugs to airway morphology.
|
