| Project/Area Number |
07670679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
YOSHIMURA Kunihiko The Jikei University School of Medicine Assistant Professor, 医学部, 講師 (60246452)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | diffuse panbronchiolitis / airway epithelium / gene expression / cystic fibrosis / CFTR gene / CC10 gene / differential display |
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| Research Abstract |
Diffuse panbronchiolitis (DPB) is a chronic inflammatory airway disease affecting mainly adults. Although pathogenesis of the disease remains unknown, genetic predisposition is suspected since the reported cases of DPB are predominantly from Asian countries such as Japan. Also, not a few similarities exit between DBP and cystic fibrosis (CF) in terms of pulmonary manifestations. First, to evaluate the possibility that DPB may be caused by abnormal expression of the gene (s) specific to airway epithelium, genomic DNA as well as mRNA extracted from airway cells (after conversion to cDNA) were analyzed for mutations of the CFTR and CC10 genes in individuals with DPB.So far, no disease-causing major CFTR gene mutations including DELTAF508 have been found in DPB individuals. Further, any pathological nucleotide substitution was not detected in the CC10 gene in DPB individuals, suggesting that the pathogenesis of DPB is not likely related to CC10 gene expression. Second, differential display analysis using airway epithelial cell mRNA obtained from DPB and normal individuals revealed several differentially expressed genes. The further investigation on these genes are currently underway.
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