| Project/Area Number |
07670684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Suzuka University of Medical Scinece and Technology |
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Principal Investigator |
KOMADA Hiroshi Suzuka University of Medical Science and Technology, Department of Clinical Nutrition, Associate Professor, 保健衛生学部, 助教授 (10144247)
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| Co-Investigator(Kenkyū-buntansha) |
TURUDOME Masato Mie University School of Medicine Department of Microbiology, Associate Professo, 医学部, 助教授 (50159042)
ITO Yasuhiko Mie University School of Medicine Department of Microbiology, Professor (00022872)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | parainfluenza virus type 4 / expressed cell / HN protein / ELISA / virus neutralization / パラインフルエンザウイルス4型 / F蛋白 / HN蛋白 / シークエンス |
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| Research Abstract |
About 250 human sera were collected, and they were analyzed by enzyme linked-immunosorbent assay (ELISA). We found that about 20 sera were positive for human parainfluenza type 4 virus (HPIV-4). The same sera had neutralization activity against HPIV-4. However, it was impossible to distinguish whether the sera inhibited HPIV-4A or 4B growth. To analyze whether the infection was caused by HPIV-4A or 4B,we established L929 cell lines which express HN proteins of HPIV-4A or 4B,because there was a big antigenic difference between HPIV-4A and 4B HN proteins. The identification is now in progress.
To elucidate the cause of the antigenic difference between HPIV-4A and 4B,3 mutated clones of 4B HN gene were constructed, and we established L929 cell lines which express the mutated HN proteins of HPIV-4B.The antigenicity was analyzed by flow cytometry using monoclonal antibodies against HPIV-4, and it was indicated that the sugar binding sites may in part contribute to the antigenic difference between HPIV-4A an 4B HN proteins.
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