| Project/Area Number |
07670688
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
NAGATA Nobuhiko University of Occupational and Environmental Health faculty of medicine, assistant professor, 医学部, 講師 (20189137)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Hisamitsu University of Occupational and Environmental Health faculty of medicine, researc, 医学部, 助手 (70271442)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | amiodarone / lung / pneumonitis / bronchoalveolar lavage / surfactant apoprotein / type II pneumocytes / rat / cytokines |
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| Research Abstract |
1) CHARACTERIZATION OF AMIODARONE PNEUMONITIS AS RELATED TO INFLAMMATORY CELLS AND SURFACTANT APORROTEIN
To charactterize the inflammation of amiodarone-induced pneumonitis, we developed a model in rats and quantified the density of inflammatory cells in BALF,and interaleolar septa (A-value) using histologic sections and graphic analyzer. In addition, lung tissues were studied using immunoperoxidase staining for surfactant apoprotein.
There was significant correlation between the density of neutrophils in BALF and the A-value at 5 (gamma=0.90, rho=0.037) and 7 weeks (gamma=0.90, rho=0.037), while significant correlation was determined between the density of lymphocytes in BALF and the A-value at 11 weeks (gamma=0.90, rho=0.037). In amiodarone-fed rats, extracellular surfactant apoprotein was noted in the alveolar space in addition to cytoplasm of large, foamy macrophages, type II pneumocytes and Clara cells, and extracellular surfactant apoprotein filled some alveoli at 9 weeks.
2) EXPRESSION OF PRO-INFLAMMATORY CYTOKINES mRNA FROM AL VEOLAR MACROPHAGES IN AMIODARONE PNEUMONITIS INDUCED IN RATS.
To investigate the role of AMs in the development of amiodarone-induced pulmonary toxicity, we induced amiodarone pneumonitis in rats and examined the expression of proinflammatory cytokines mRNA from AMs.
Expression of IL-1 alpha mRNA was noted after 1 week and significantly increased after 5 weeks of feeding, while increased expression of TNF alpha mRNA was observed only after 1 week of feeding. Expression of CINC-1 mRNA was increased after 1 and 5 weeks of feeding.
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