| Project/Area Number |
07670699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TODA Tatsushi Institute of Medical Science University of Tokyo, Associate Professor, 医科学研究所, 助教授 (30262025)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Fukuyama-type congenital muscular dystrophy (FCMD) / chromosome 9q31 / linkage disequilibrium / founder-haplotype / genetic diagnosis / prenatal dianosis / brain anomaly / ポジショナルクローニング / 9番染色体長腕31 / 連鎖不平衡 / コスミドコンティグ / 創始者染色体 / 9番染色体長腕引 / 連鎖不平衡マッピング / 酵母人工染色体 / glia limitans |
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| Research Abstract |
Fukuyama-type congenital muscular dystrophy (FCMD), the second most common form of muscular dystrophy in Japan, is an autosomal recessive severe muscular dystrophy, associated with brain anomalies.
1. Following our initial mapping of the FCMD locus to chromosome 9q31-33, we found linkage disequilibrium between FCMD and mfd220 on 9q31 and then constructed the YAC contig encompassing mfd220.
2. By using linkage-disequilibrium mapping, we narrowd the candidate region to <100 kb containing D9S2107 and constructed the consmid contig harboring D9S2107.
3. We examined haplotypes of FCMD chromosomes at a few loci around D9S2107. The results indicated that 80% of FCMD-bearing chromosomes carried an ancestral haplotype and that 95% of FCMD patients carried ancestral haplotypes homozygously or heterozygously. There were only a few haplotypes other than the founder one. We predicted the gene location extremely proximal to marker E6 by founder-haplotype mapping.
4. We screened genomic rearrangements in FCMD using each clone of the cosmid contig around D9S2107 as a probe. A -3 kb insertion was found near the marker E6, which lies -50 kb proximal to D9S2107, in most FCMD chromosomes with the founder haplotype (86%). The frequency of this insertion in normal chromosomes matched well that of FCMD carrier.
5. We performed prenatal dianoses of 10 and several FCMD familes. All the results were correct. Also, we conducted genetic diagnosis of 30-40 families. We demonstrated that breaches in the glia limitans may be the primary cause of the micropolygyria in FCMD by pathological study of an FCMD fetus.
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