| Project/Area Number |
07670708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
TAKAGISHI Yoshiko Nagoya University, Research Institute of Environmental Medicine (RIEM), Research Associate, 環境医学研究所, 助手 (50024659)
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| Co-Investigator(Kenkyū-buntansha) |
KANOU Yasuhiko Nagoya Univ., RIEM,Research Associate, 環境医学研究所, 助手 (50252292)
NAGAYA Takashi Nagoya Univ., RIEM,Research Associate, 環境医学研究所, 助手 (80262913)
FUNAHASHI Atsushi Nagoya Univ., School of Informatics and Science, Associate Professor, 情報文化学部, 助教授 (10190125)
ODA Sen-ich Nagoya Univ., School of Agricultural Science, Associate Professor, 農学部, 助教授 (60023660)
INOUYE Minoru Nagoya Univ., RIEM,Associate Professor, 環境医学研究所, 助教授 (20090425)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Crerebellum / Endoplasmic reticulum / Purkinje cells / Calucium / Ataxia / Mutant / Neuronal plasticity / Rat |
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| Research Abstract |
An ataxic mutant rat, dilute-opisthotonus (dop), was originally discovered in a breeding colony of Wister rats and shows a dilution of coat color and neurological disorders with convulsivelimb movements and opisthotonic seizures continuing until the death of animals at 3 weeks of age. The animals have been bred in our laboratory by intercrossing the heterozygous (dop/+) rats. The dilute-opisthotonus gene was mapped on chromosome 8 by PCR-amplified microsatellite markers, which showed a linkage of Apoc3, dop and Mylclv genes, suggesting that this mutation was homologous to dilute-lethalof the mouse.
Electronmicroscopy and immunocytochemistry using anti-inositol 1,4,5-triphosphate (InsP3) receptor antibody revealed that smooth endoplasmic reticulum (SER) was missing in the dendritic spine of Purkinje cell in the dop cerebellum. Observation of developing Purkinje cells showed that SER was formed in the soma and dendrites but not in the spine of the Purkinje cell.
SER is assumed to be an intracellular Ca store and it has been suggested that a Ca signaling process at the synaptic site has a key role in synaptic transmission. Thus, the dop rat is thought to have impaired synaptic regulation. Electronmicroscopy and Immunocytochemistry using antibody raised against GABA,transmitter released by Purkinje cell terminals, indicated that Purkinje cell axon terminals normally contacted the neurons in deep cerebellarnuclei.
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