| Project/Area Number |
07670760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | UNIVERSITY of TOKYO |
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Principal Investigator |
NAKAJIMA Toshiaki Faculty of Medicine Assistant, 医学部・附属病院, 助手 (50227790)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAWA Kuniaki Faculty of Medicine Assistant, 医学部・附属病院, 医員
HAZAMA Hisanori Faculty of Medicine Assistant, 医学部・附属病院, 医員
挾間 比佐徳 東京大学, 医学部・附属病院, 医員
浜田 栄治 東京大学, 医学部(病), 医員
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | vascular smooth muscle / ionic currents / vasopressin / esdothelin-1 / non-selective cation channel / Mg^<2+> / fatty a cids / Ca^<2+> activated Cl^- current / vascular smooth muscle / vasopressin / non-seledue cation channel / Ca^<2+>-activated Cl^- current / Ionic currents / non-selective cation channels / voltage-dependent Ltype / trachoal smooth muscle Ca^<2+> current |
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| Research Abstract |
1) To clarify the significance and characterization of Ca^<2+> -permeable nonselective cation channels activated by vasopressin and endothelin-1 (receptor-mediated non-selective cation channels) in vascular smooth muscle cells, the patch clamp techniques, Ca^<2+> or tension measurement were applied in aortic smooth muscle cells. The results suggested that calcium ectry and membrane depolarization elicited by these agonists are partly mediated by a receptormediated Ca^<2+> -permeable non-selective cation channel in vascular smooth muscle cells. Also, we showed the characteristics and activation mechanisms of the channel. Furthermore, we investigated the effects of extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids, which are known to produce vascular relaxation or hypotensive effects, on the receptor-mediated non-selective cation channels. We showed that extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids effectively inhibit receptor-mediated non-selective cation channel. which may play an important role in the regulation of vascular tone.
2) In tracheal myocytes, to investigate the activation mechanism and characterization of Ca^<2+> -dependent Cl^- current, the patch clamp technique was applied. We presented that Ca^<2+> -dependent Cl^- current can be activated by SR (sarcoplasmic reticulum) Ca^<2+> release due to neurokinin A or caffeine (through IP_3 or ryanodine receptors) as well as by Ca^<2+> infulx due to the voltage-dependent L-type Ca^<2+> current. It also showed that the functional coupling of ryanodine receptor to the voltage-dependent L-type Ca^<2+> channels exists in tracheal smooth muscle cells.
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