| Project/Area Number |
07670765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | TOKYO MEDICAL & DENTAL UNIVERSITY |
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Principal Investigator |
HIROE Michiaki TOKYO MEDICAL & DENTAL UNIVERSITY,SECOND DEPARTMENT OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 講師 (80101872)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Hiroshi TOKYO MEDICAL & DENTAL UNIVERSITY,SECOND DEPARTMENT OF MEDICINE,ASSISTANT PROFES, 医学部, 助手 (10232464)
ISHIYAMA Shigeru TOKYO MEDICAL & DENTAL UNIVERSITY,SECOND DEPARTMENT OF MEDICINE
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | myocyte cell death / apoptosis / myocarditis / myocardial infarction / Fas / Fas ligand / lymphocyte / 心筋障害 |
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| Research Abstract |
There exist two general types of cell death, necrosis and apoptosis, marked by different morphological and molecular characteristics. The mechanism of the cardiomyocyte dath has been classicaly believed to be necrosis in various heart diseases. Therefore, we examined whether apoptotic cardiomyocyte death occurs in acute fulminant myocarditis and scute myocardial infarction and if so, whether the Fas (CD95)/Fas ligand system is associated with the apoptosis. Nucleosomal DNA fragmentation, the biologic hallmark of apoptosis, was detected in numerous cardiomyocytes of these diseases. The expression of Fas antigen protein was upregulated in some of cardiomyocytes, but not in the control. In myocarditis, the expression Fas ligand mRNA and protein was expressed in some of CD4+and CD8+ T cells. Co-expression of Fas and Fas-L was observed in some infiltrating lymphocytes. These results indicate that there exist apoptotic death of the affected cardiomyocytes and infiltrating lymphocytes in acute fulminant myocarditis and myocardial infarction. It is likely that the apoptosis of cardiomyocytes might be associated with the Fas/Fas-ligand pathway.
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