| Project/Area Number |
07670883
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
NUMAZAKI Kei Sapporo Medical University School of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (00228272)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAI Shuji Sapporo Medical University School of Medicine, Department of Pediatrics, Instruc, 医学部, 助手 (30230907)
NAKATA Shuji Sapporo Medical University School of Medicine, Department of Pediatrics, Assista, 医学部, 講師 (70155745)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | cytomegalovirus (CMV) / breast-feeding / reactivation / cytokines |
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| Research Abstract |
Breast-feeding is the major factor of vertical transmissions during the first year of life in countries where the majority of women were seropositive to human cytomegalovirus (CMV) and breast-feed their infants. Studies in our and other laboratories provided compelling evidence that virolactia was more common in breast milk samples collected during 1 week to 3 months postpartum than in those collected during the first week. We collected colostrum and breast milk from 65 human anti-CMV lgG antibody-positive mothers who breast-fed at 3 days and a month after delivery. When investigated by indirect immunofluorescence assay (IFA), liquid supernatant of colostrum without cytotoxicity was not found to exert inhibitory effect on HCMV-infected MRC-5 cells. The activities of tumor necrosis factor (TNF)-alpha were significantly higher in HCMV DVA-negative colostrum than DNA-positive breast milk (p<0.05). Serum levels of cell free soluble interleukin-2 receptor (slL-2R) of mothers of DNA-positive milk at one month after delivery were significantly higher than those of mothers of DNA-negative milk (p<0.05). It is likely that levels of cytokines such as slL-2R in serum are related to reactivation of HCMV which occurs lo cally in the mammary gland of the lactating mother after delivery.
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