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Expression of the Id helix-loop-helix proteins in human myeloid and lymphoid cells

Research Project

Project/Area Number 07670899
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionTeikyo University

Principal Investigator

ISHIGURO Akira  Teikyo university, School of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (90222984)

Co-Investigator(Kenkyū-buntansha) INABA Yuji  Teikyo university, School of Medicine, Department of Pediatrics, Medical Staff, 医学部, 助手 (70266310)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsId / helix-loop-helix protein / transcription factor / differentiation / mycloid leukemia / lymphocytes / human T-cell leukemia virus / leukemia / HTLV-1 / differentiation / myeloid leukemia
Research Abstract

Id proteins are helix-loop-helix transcriptional factors that lack the basic DNA binding domain. The Id proteins have been generally reported to function as an inhibitor of cell differentiation. We examined the expression of mRNAs encoding human Id1, Id2 and Id3 by Northern hybridization in human mycloid and lymphoid cells. Mycloid leukemia cells with monoblastic and crythroid characteristics expressed Id2 mRNA.The levels of Id2 mRNA markedly increased with induction of differentiation of the mycloid blasts toward both granulocytes and macrophages. Increascs in Id2 mRNA expression were found in more differentiated blasts in fresh AML samples. In normal mycloid cells, Id2 mRNA was expressed cultured macrophages from bone marrow, and in mature granulocytes and monocytes from peripheral blood. Only small numbers of mycloid cells expressed mRNAs for Id1 (K562 crythroid mycloid cells) and Id3 (U-937 and THP-1 monoblastic cells). In contrast, cells from B-and T-cell lines expressed Id2 and/or Id3 mRNAs. Human T-cell leukemia virus-transformed cells prominently expressed only Id2 mRNA.Upon PHA-stimulation of blood normal lymphocytes, Id2 expression decreased and Id3 mRNA levels were up-regulated. These results show that Id2 protein may contribute to mycloid differentiation, and both Id2 and Id3 proteins may be involved in a pathway that controls lymphoid activation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Ishiguro A: "Expression of Id2 and Id3 mRNA in human lymphocytes" Leukemla Res. 19. 989-996 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Reference intervals for serum granulocyte colony-stlmulating factor levels in children" J Pediatr. 128. 208-212 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Id2 expression increases with myeloid differentlation in human myeloid cells" Blood. 87. 5225-5231 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Expression of the Id helix-loop-helix proteins in human myeloid and lymphoid cells" Exp Hematol. 24. 1113 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Production of interleukin-10 in the cerebrospinal fluid in aseptic menigitls" Pedlatr Res. 40. 610-614 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukushima K: "Transient elevation of granulocyte colony-stlmuiating factor levels in cerebrospinal fluid at the initial stage of aseptic meningitls in children" Pedlatr Res. 37. 160-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Spirin K: "Identification of the helix-loop-helix proteins expressed in myeloid cells by moiecuiar cloning" Blood. 86. 682a (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Effects and serum levels of thrombopoietin in a case of chronic thrombocytopenia with achondroplasia" Int J Haematol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba Y: "The production of macrophage inflammatory protein-1α in the cerebrospinal fluid at the initial stage of meningitls of children" Pediatr Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石黒 精: "先天性好中球減少症と遺伝子異常" 血液・腫瘍科. 30. 235-240 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石黒 精: "白血球計数,分画,形態." 小児の保健治療指針(小児科診療増刊号). 59. 5-7 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 稲葉雄二: "Ubenimexが急性白血病児のInterleukin-6産生能におよぼす作用の検討" solotherapy. 9. 397-398 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐藤敦子: "メソトレキセート大量療法後に腎不全を呈した骨肉腫の1例" 小児科臨床. 59. 1227-1230 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池田紀子: "気管支喘息児の日常生活:鍛練療法と自律神経症状に関するアンケート調査" 日児アレルギー誌. 10. 448-453 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池田紀子: "気管支喘息児の自律神経症状についての多施設アンケート調査:自律神経症状と喘息の重症度および血清IgE値" 日児アレルギー誌. 10. 478-481 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石黒 精: "Annual Review血液1995" 中外医学社, 327 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石黒 精: "小児疾患のインフォームド・コンセント" 診断と治療社, 341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新保敏和: "改訂CD分類ハンドブック(1995)-細胞膜分化抗原の国際分類" 癌と化学療法社, 677 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐藤敦子: "副作用症例データベース" 診断と治療社, 341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Spirin K,Shiohara M,Tobler A,Norton JD,Rigolet M,Shimbo T,Koeffler HP: "Expression of Id2 and Id3 mRNA in human lymphocytes." Leukemia Res. 19 (12). 989-996 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Inoue K,Nakahata T,Nishihira H,Kojima S,Ueda K,Suzuki Y,Shimbo T: "Reference intervals for serum granulocyte colony-stimulating factor levels in children." J Pediatr. 128 (2). 208-212 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Spirin K,Shiohara M,Tobler A,Gombart F,Istael MA,Norton JD,Koeffler HP: "Id2 expression increases with myeloid differentiation in human myeloid cells." Blood. 87 (12). 5225-5231 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Spirin K,Shiohara M,Koeffler HP,Shimbo T: "Expression of the Id helix-loop-helix proteins in human myeloid and lymphoid cells." Exp Hematol. 24 (9). 1113 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Suzuki Y,Inaba Y,Komiyama A,Koeffler HP,Shimbo T: "Production of interleukin-10 in the cerebrospinal fluid in aseptic menigitis." Pediatr Res. 40 (10). 610-614 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukushima K,Ishiguro A,Shimbo T: "Transient clcvation of granulocyte colony-stimulating factor levels in cerebrospinal fluid at the initial stage of aseptic menigitis in children." Pediatr Res. 37 (2). 160-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Spirin K,Shiohara M,Ishiguro A,Seliverstov AI,Koeffler HP: "Identification of the helix-loop-helix proteins expressed in myeloid cells by molecular cloning." Blood. 86 (suppl 1). 682a (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A,Nakahata T,Muraoka K,Thabara T,Miyazaki H,Kato T,Inaba Y,Shimbo T: "Effects and sctum levels of thrombopoictin in a case of chronic thrombocytopenia with achondroplasia." Int Haematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba Y,Ishiguro A,Shimbo T: "The production of macropage inflanmatory protein-1 alpha in the cerebrospinal fluid at the initial stage of meningitis of children." Pediatr Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishiguro A: "Reference intervals for serum granulocyte colony-stimulating factor levels in children" J Pedlatr. 128(2). 208-212 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishiguro A: "Id2 expression increases with myelold differentiation in human myeloid cells" Blood. 87(12). 5225-5231 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishiguro A: "Expression of the Id hellx-loop-hellx proteins in human myeloid and lymphoid cells" Exp Hematol. 24(9). 1113- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishiguro A: "Production of interleukin-10 inthe cerebrospinal fluldi n aseptle menigitis" Pediatr Res. 40(10). 610-614 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishiguro A: "Effects and serum levels of thrombopoletin in a case of chronic thrombocytopenla with achondroplasia" Int J Haematol (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Inaba Y: "The production of macrophage inflammatory protein-1 α in the cerebrospinal fluid at the initial stage of meningitis of children" Pediatr Res (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 石黒精: "白血球計数,分画,形態." 小児の保健治療指針(小児科診療増刊号). 59. 5-7 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐藤敦子: "メソトレキセート大量療法後に腎不全を呈した骨肉腫の1例" 小児科臨床. 59(7). 1227-1230 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 池田紀子: "気管支喘息児の日常生活:鍛練療法と自律神経症状に関するアンケート調査" 日児アレルギー誌. 10(4). 448-453 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 池田紀子: "気管支喘息児の自律神経症状についての多施設アンケート調査:自律神経症状と喘息の重症度および血清IgE値" 日児アレルギー誌. 10(4). 478-481 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石黒精: "死体解剖.小児疾患のインフォームド・コンセント" 診断と治療社, 341 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新保敏和: "改訂CD分類ハンドブック(1995)-細胞膜分化抗原の国際分類" 癌と化学療法社, 677 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐藤敦子: "副作用症例データベース" 診断と治療社, 1 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukushima K, Ishiguro A, et al.: "Transient elevation of granulocyte colony-stimulating factor levels in cerevrospinal fluid at the initial stage of aseptic meningitis in chidren." Pediatr Res. 37. 160-164 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 稲葉雄二,石黒 精など: "Ubenimexが急性白血病児のinterleukin-6産生能におよぼす作用の検討." Biotherapy. 9. 397-398 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Spirin K, Ishiguro A, et al: "Identification of the helix-loop-helix proteins expressed in myeloid cells by molecular cloning." Blood. 86(suppl 1). 682a

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishiguro A, et al: "Reference intervals for serum granulocyte colony-stimulating factor levels in children." J Pediatr. 128. 208-212 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishiguro A, et al: "Expression of Id2 and Id3 mRNA in human lymphocytes." Leukemia Res. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 石黒 精など: "先天性好中球減少症と遺伝子異常." 血液・腫瘍科. 30. 235-240 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石黒 精など: "Annual Review 血液1995 高久史麿など(編)" 中外医学社, 327 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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