| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Research Abstract |
To investigate the dorsal and ventral aspects of normal human nails in scanning electron microscopy, the keratinocytes piled up each other in the dorsal and ventral surface, and the dorsal and ventral aspects showed a flat pattern and a wave shape of longitudinal grooves respectively. On the other hand, the dorsal and ventral aspects of nails of psoriasis vulgaris had many slits between the cells, so it showed a rough surface.
X-ray analysis revealed that the dorsal aspect was about 2 times the sulfur rate of the ventral one. However, the dorsal and ventral aspects of nails of psoriasis vulgaris had almost equal levels of sulfur rates. To count the sulfur values as dot patterns in X-ray analysis, the horny and glanular layrs were denser than those of the squamous and basal layrs in the normal epidermis. Howerver, all layrs of the epidermis of psoriasis vulgaris had a similar density, while the epidermis of lichen planus showed a same change of dot pattern as the nomal epidermis. On the
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other hand, the dot density of the basal layrs was lighter than that of squamous, glanular, lucid and horny layrs, which had equal dense patterns, in palmoplantar keratoderma (Unna-Thost type).
When we have analyzed calcium values in the normal epidermis, we have known the presence of dystrophin, which is one of calcium-binding proteins. Then the localization of dystrophin in the epidermis was investigated by immunofluorescence and immunoelectron microscopic studies using monoclonal antibodies against dystrophin. The dystrophin was localized in the attachment plaques of desmosomes of epidermal keratinocytes, but not in those of hemidesmosomes of epidermal basal cells. The epidermis of psoriasis vulgaris had a similar pattern of immunofluorescence staining as the normal epidemis. Moreover, the dystrophin was localized in the intercellular portions of basal cell carcinoma and squamous cell carcinoma. In immunofluorescence studies of both cancers, the more keratinized cells had denser staining patterns of dystrophin. Less
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